Maytenus putterkloides has been used traditionally to manage several diseases including diabetes, however, its efficacy has not been scientifically evaluated. The aim of this study was to determine the in vivo hypoglycemic activity of aqueous and ethyl acetate leaf extract of this plant in male swiss white albino mice. The antidiabetic activity was screened in alloxan induced diabetic mice through oral and intraperitoneal routes. Phytochemical composition was assessed using standard procedures. The aqueous and ethyl acetate leaf extract of this plant showed hypoglycemic activity at dose levels of 50, 100, 200, 300 mg/kg body weight in a dose independent manner via both routes. The extracts contained total phenolics, saponins, tannins, flavonoids, alkaloids and phytonutrients; vitamins B1, B3, and C. The observed hypoglycemic activity could be associated with the phytochemicals present in this plant extract.