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Since the cell walls of basidiomycetes consist of chitin and other things,1} chitinolytic enzymes seem to be essential for protoplast formation. There are considerable numbers of reports on the formation of basidiomycete protoplasts, but only one on Tricholoma matsutake.2) Chitinase was effective for protoplast formation from Lentinus edodes^and other basidiomycetes in the presence of Cellulase Onozuka RS. Furthermore, Trichoderma harzianum isolated from L. edodes logs produced chitinase and /?-l,3-glucanase, and these enzymes were effective for protoplast formation from L. edodes and somebasidiomycetes.40 Similar results were obtained in protoplast