The aimof this research was to evaluate the in vitro anticoccidial activity of microencapsulated essential oils from Lepechinia heteromorpha(Briq.) Epling and Salvia sagittataRuiz & Pav., against non-attenuated sporulated oocysts of Eimeriasppof broiler chickens. The treatments were the concentrations of the microencapsulated in 2.5; 5; 10; 20;30 and 50 mg/ml, 2.5% potassium dichromate negative control group and 4% formaldehyde positive control group. The destruction of oocysts in liquid medium was evaluated by microscopic counting and the results were verified after 24 and 48 h of incubation at 28 °C. Data were analyzed by analysis of variance, using SPSS version 25 software and Tukey's test. The results showed highly significant statistical differences between the microencapsulated, as well as in their concentrations, registering the greatest destruction of sporulated oocysts by the microencapsulated essential oil of Salvia sagittataRuiz & Pav. at a concentration of 50 mg/ml at 48 h of incubation, reaching83.5% destruction of oocysts, this value being similar to 4% formaldehyde. Concluding that the microencapsulated essential oils studied destroy up to 83.5% of the sporulated oocysts of Eimeriaspp.
This study evaluated the chemical composition and anti-proliferative activity of essential oils (EOs) obtained by hydrodistillation from seven medicinal plants from Cachicadán, La Libertad Region, Peru. Limonene (0.64 to 44.43%) and linalool (0.36 to 2.12%) were identified in all EOs by gas chromatography coupled to mass spectrometry analysis. The major components (relative intensity ≥ 10%) were cisdihydro carvone, carvone, and cis-piperitone epoxide for Minthostachys mollis leaves; β-pinene, limonene, and ledol for Lepechinia heteromorpha leaves; limonene, neral, and geranial for Aloysia citriodora, both leaves and flowers; αpinene, and limonene for Myrcianthes myrsinoides leaves; and α-pinene, βmyrcene, and (E)-β-Ocimene for Dalea carthagenensis leaves. Constituted by (Z)β-ocimene, dihydrotagetone, (Z)-tagetone, and car-3-en-2-one, EO of Tagetes minuta leaves induced an irreversible cytostatic effect against MCF-7 human breast tumour cells. Further in vivo studies must be carried out to establish the safe and efficient dose of T. minuta EO as adjuvant treatment in oncological therapies.
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