No abstract

Anti-cancer biomarker aptamers are not always well characterized leading to unreliable performance in analytical assays  Fast isothermal rolling circle amplification (15 min) leads to a 10 3 -fold increase in the functional affinity of the aptamer  Most favorable interaction on the solid-surface due to increase in the local protein concentration  Different stability of aptamer-free protein and aptamer-bound protein complexes compromise the efficiency of competitive assays *Highlights (for review)No RCA With RCA