The objective of this study was to evaluate the occurrence of anti-Leptospira spp. antibodies in female buffalo in the state of Pernambuco. A total of 123 female buffalo blood samples were collected from five properties distributed in the state of Pernambuco. The microscopic agglutination test was used to study anti-Leptospira spp. antibodies. The occurrence of anti-Leptospira spp. antibodies was 28.5% (35/123; CI 20.7–37.3%) and on different properties, the occurrence ranged from 28.6% to 80.0%, with 100% of the properties showing animals with positive results. The serovars of the serogroup Sejroe with a higher incidence were Hardjoprajtino (CTG strain, 49.1%) and Hardjo (Prajtino genotype, 43.2%), followed by serogroup Grippotyphosa with the Grippotyphosa serovar (3.9%), serogroup Pomona with the Pomona serovar (1.9%), and the Icterohaemorrhagiae serovar Copenhageni (1.9%). This was the first record of the occurrence of anti-Lepstospira spp. antibodies in female buffalo in the state of Pernambuco. Control measures are necessary to prevent health and economic losses, given that the agent involved affects animal reproduction, triggering drops in conception rates or even clinical cases of abortion.

This study aimed to determine the prevalence and risk factors associated with Leishmania spp. and Trypanosoma cruzi infections in dogs presented at veterinary clinics in the city of João Pessoa, Paraíba state, northeastern Brazil. A total of 384 dogs from 34 veterinary clinics were sampled between April 2015 and May 2016. For serological diagnosis of canine visceral leishmaniasis and Chagas disease, the indirect fluorescent antibody test was used. Reactive sera were submitted to a confirmatory test using the S7 ELISA. The seroprevalence of Leishmania spp. was 2.8% (11/384), and that of T. cruzi was 1.5% (6/384). Two animals were positive for both parasites. The variable “access to the street” was identified as a risk factor for Leishmania spp. infection (OR = 4.81; 95% CI = 1.23–18.75). We concluded that a significant proportion of dogs presented at veterinary clinics in João Pessoa are seropositive for Leishmania spp. or T. cruzi infections, and we suggest that greater care be taken with dogs that have access to the street.

Rabies is a zoonosis of extreme importance to public health. Specifically in herbivores it causes economic losses, considering its lethality. The diagnosis provides information to describe the epidemiology of the disease in the regional study. For this purpose, a survey of Central Nervous System (CNS) materials, received for diagnosis in the Center-West region of the State of São Paulo, by the Zoonosis Diagnostic Service (SDZ), Faculdade de Medicina Veterinária e Zootecnia (FMVZ)-UNESP-Botucatu-SP. In the period from 2001 to 2015, a total of 643 samples were analyzed, being 489 (76.05%) of the bovine species, with 68 (13.9%) positive samples and 154 (23.95%) of the equine species with 17 (11.3%) positive. For the analyzed bovine samples, sensitivity and specificity values of the fluorescent antibody test (FAT) were 88.24 and 99.52% respectively and for equines, 47.06 and 100.0%. The frequency of samples received by species, month, and year and the results of the diagnostic tests recommended by the World Health Organization (WHO), fluorescent antibody test (FAT) and the mouse inoculation test (MIT), were analysed.