Objective: To determine the resistance profiles of bacterial pathogens isolated in a hospital institution in Montería -Córdoba. Methodology: An observational study was carried out, during the months of January to December 2018; 344 samples were evaluated. Mention about the source of study samples. The identification and susceptibility of the bacteria were determined through the VITEK system. Findings: Among the Gram-negative bacteria identified were Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli, Acinetobacter baumannii and Serratia marcescens, while Gram-positives were the Staphylococcus epidermidis, S. aureus, S. haemolyticus and Enterococcus faecalis. Gram-negative isolates such as Klebsiella pneumoniae showed resistance to piperacilinatazobactam, ceftazidima, cefepima, doripenem, imipenem, meropenem, amikacina and ciprofloxacina, being sensitive to gentamicin. P. aeruginosa, E. coli and A. baumannii showed resistance to all antimicrobials, with the exception of colistin. S. marcescens only showed resistance to ceftazidime. Enterobacter cloacae showed resistance to most drugs, with the exception of amikacin and gentamicin. Among the Gram-positive isolates, S. epidermidis showed resistance to Trimethoprim / Sulfamethoxazole, erythromycin, nitrofurantoin, ciprofloxacin, and levofloxacin. S. aureus and Staphylococcus haemolyticus showed susceptibility to all antimicrobials, while E. faecalis and Staphylococcus hominis only showed resistance to erythromycin. Conclusion: A wide prevalence of antimicrobial resistance (mention the milieu) was observed in this investigation.