Introduction: This work is part of the UNAM-DGAPA-PAPIIT Project IN304521, ENEO No124 entitled "Educational intervention to prevent gender violence in university nursing students: design and evaluation", which was approved by the ethics and research committee of the ENEO-UNAM. Objective: Build and validate a documentary measurement instrument for gender violence in nursing university students. Methodology: Based on the Supo taxonomy. Design and validation of instruments type documentary measurement, of multidimensional content to measure the perception, attitudes, knowledge and gender violence in Mexican university nursing students. The content was built from a literary search on the web, the last 10 years. The content validation was through rational validity and judging of 8 gender experts. The metric properties were determined by Variances and Cronbach's Alpha from the application of a pilot test with 80 students from various nursing schools and semesters, they were selected by non-probabilistic convenience sampling. The sample was calculated with a confidence level of 0.95% and a probability of 0.05%. The collection method was remote. Informed consent was requested, including clarification of doubts, associated risks and benefits, as well as data protection. Processing in statistical software SPSS 21. Results: The judges evaluated the content as sufficient (95.10%), clear (96.57%), pertinent (96.90%) Relevant (85.13%) and with applicability (95.93%). By general consensus the content validity was 93.93%. The construct validity was ratified with the strength of heterogeneity of the variances other than zero in all dimensions. The instrument obtained very high reliability (Cronbach .945). The conclusive instrument was constructed with 80 items distributed in four dimensions: perception, attitudes, knowledge and gender violence with 5 likert-type response options; an inventory with 5 additional questions and a section for the characterization of the sample. Approximate reasonable application time of 15 min. Conclusions: Based on the test theory, the documentary measurement instrument has a high precision to measure the gender violence variable.