This study was designed to determine the effect of Quran listening without its musical tone (Tartil) on the mental health of personnel in Zahedan University of Medical Sciences, southeast of Iran. The results showed significant differences between the test and control groups in their mean mental health scores after Quran listening (P = 0.037). No significant gender differences in the test group before and after intervention were found (P = 0.806). These results suggest that Quran listening could be recommended by psychologists for improving mental health and achieving greater calm.