Four potential inhibitors, hydroxymethylfurfural, furfural, syringaldehyde and vanillin, were assayed over a range of concentrations and times to simulate the amounts that might be expected to accumulate when the hydrolyzed water-soluble fraction from steamtreated aspenwood was added in a fed-batch mode to K. pneumoniae. Combinations of the individual materials resulted in a cumulative inhibitory effect. Prolonged incubation could alleviate some of the inhibition.
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