No abstract

Immunoassays are analytical methods that achieve the detection and quantification of analytes, particularly peptides and proteins in biological samples, through the formation of a stable complex between the analyte and a specific antibody. They represent very selective and sensitive techniques that have found application in several areas such as clinical chemistry, bioanalysis, pharmaceutical analysis, toxicological analysis, and environmental analysis. [1][2][3] Owing to their capacity for high throughput and significantly reduced average analytical times, through the simultaneous analysis of numerous samples with ultimate detection sensitivity, immunoassays are the preferred platform for most protein studies, particularly clinical diagnostics and drug development where specificity is critical. 4-6 Among various immunoassays, the most commonly employed in routine clinical settings are the enzyme-linked immunosorbent assay (ELISA)