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A sensitive method is described for the determination of riboflavin (RF), flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) in serum of different fish using reversed-phase high-performance liquid chromatography (HPLC) with fluorimetric detection. Trichloroacetic acid was used to isolate B2 vitamers from the serum, and an aliquot of this solution was analysed by HPLC with a Zorbax-NH2 column with methanol -0.2 M phosphate buffer (1 + 9) as the mobile phase. The detection limits of RF, FMN and FAD in the serum were 4.89, 9.13 and 73.1 ng ml-1, respectively.

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