Plasma fibrinolysis in rats rose above the level of physiological fluctuations in a
curve with two peaks at 1 and 6 h, following intraduodenal administration of high-molecularweight
urokinase (HMW-UK; MW 53,000; 124,000 IU/mg protein). Activation of plasma
fibrinolysis was also confirmed with insolubilized enzyme (glass-coupled UK), but lacked the
first activity peak. Plasma fibrinolytic enzyme isolated by affinity chromatography revealed
strong fibrinolytic (1,120 IU/dl), pyro-Glu-Gly-Arg-pNA amidolytic (3,200 nmol/dl) and
Glu-plasminogen activating (24.5 IU/dl) activities. Using specific UK antibody, it appeared
that the first peak originated from the administered UK, while the second one derived from
endogenous plasminogen activator. Dose response of UK was not observed, and the maximal
effect was at about 5,000 IU/kg body weight.
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