In view of the increasing importance of chitosan, this study was carried out to investigate the preparation and characterization of chitosan from chicken feet which were economically unevaluated. Chitosan was prepared from the feet of chicken using the conventional methods of pretreatment, demineralization, deproteinization, discoloration (chitin), and deacetylation (chitosan). The yield of crud chitin and chitosan were 17% and 5% respectively. The results obtained revealed that molar mass average of chitosan monomer was 165.62 g/mol with degree of deacetylation (DDA) 89% calculated from mathematical and UV spectrophotometer methods. The FT-IR spectrum of chitosan also determined and characterization was done and compared with standards.