& Accurate, sensitive, and precise stability indicating TLC-Densitometric method was developed and validated for determination of chlorpropamide and its main degradation product and related impurity; 4-Chlorobenzenesulphonamide. The method is based on chromatographic separation on silica gel 60 F 254 TLC plates using chloroform: ethyl acetate: triethylamine: glacial acetic acid (70:30:3:1, by volume) as a developing system followed by densitometric measurements at 230 nm. Chlorpropamide was subjected to acid and alkaline hydrolysis, oxidation, and photodegradation. Method validation was carried out according to ICH guidelines and the proposed method was successfully applied to the analysis of chlorpropamide in pharmaceutical dosage form where no interference from additives has been found. Results obtained by the proposed method was statistically compared with those obtained by the reported RP-HPLC method and no significant difference.

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