Objective: The purpose of this study was to analyze the effectiveness of Tetragonula sapiens propolis collected from North Luwu, Sulawesi, Indonesia to treat anemia in adolescent girls. Methods: A quasi-experimental design with a two-group pre-test—the post-test design was applied, and 44 respondents were chosen by using purposive sampling. The respondents were divided into two groups. Each group was given iron supplement every day. For the intervention group, the respondents consumed 5 drops of propolis for three times a day. Meanwhile, for the control group, IPI vitamin C was given instead of propolis. The intervention was given for 30 d in a row. The hemoglobin (Hb) level measurement was conducted after 30 d. Results: The results showed that the Hb levels of the intervention group were higher compared to the control group after propolis was given for 30 d. The result of the analysis showed a significant value of (2-tailed) 0.000<0.05. Conclusion: Tetragonula sapiens propolis could be used to increase the Hb levels in anemic adolescent girl.