Quantum Dots (QDs) are ideal fluorescent labels for lateral flow assays (LFA) due to their unique optical properties and resistance to chemical and photo-degradation. Lateral flow assay, known as immunochromatography test, is a diagnostic strip test that uses paper or membrane-based devices to detect the presence/absence of an analyte with the pregnancy test, the most known LFA. Diagnostic tools for non-communicable (NCDs) and communicable diseases (CDs) are available. However, other NCDs and CDs do not have LFAs as first-line diagnosis/point-of-care. QDs are promising fluorescent labels as they improve the LFA’s colourimetric sensitivity and fluorescence stability. This chapter discusses the tailoring and application of QDs in LFA. Particular focus on the applications of QDs in LFA for detecting or screening NCDs (i.e. cancers etc.) and CDs (i.e. SARS-CoV-2, HIV/AIDs etc.).The book chapter concludes by discussing different challenges and perspectives of QDs in LFA.