The research purpose was to evaluate the influence of date extract supplementation in lactate ringer-egg yolk extender with 0.025% sodium dodecyl sulfate (LREYS) on post-thawing quality of Pelung chicken spermatozoa. Completely randomized design with 5 treatments and 4 replications was used in this study. Semen was collected from three Pelung chickens once in three days using the dorsal-abdominal massage method. Semen was divided into 5 treatment groups of date extract supplementation that were 0% as control (LREYSDE0), 0.5% (LREYSDE1), 1% (LREYSDE2), 1.5% (LREYSDE3), and 2% (LREYSDE4). The post thawing liquid semen was observed for sperm motility, viability, plasma membrane integrity and acrosome integrity. The result showed that the motility, viability, membrane plasma integrity and acrosomal integrity of spermatozoa in LREYSE extender with 1.5% date extract supplementation (30.44±1.02%; 49.83±0.91%; 43.26±1.02%; and 45.33±0.72% respectively) was significantly higher (P0.05) than others treatment that were 0% (18.21±0.43%; 35.62±0.51%; 30.54±0.60%; and 31.49±0.71% respectively), 0.5% (20.45±0.72%; 40.72±0.87%; 36.81±0.55%; and 38.56±0.63% respectively), 1% (25.68±0.93%; 46.27±0.75%; 40.52±1.02%; and 42.83±1.09% respectively) and 2% (22.55±0.92%; 43,71±0,74%; 38,84±0,71%; and 41,39±0,86% respectively) of date extract supplementation. It is concluded that the 1.5% date extract supplementation in extender can maintain the best post-thawing sperm quality.

Duck is one of the commodities that contribute to the national livestock production and Central Java is home to the fourth biggest duck population after West Java, South Sulawesi and East Java. The 2019 egg production in Central Java was 36.174 tons or 11.3% of the total egg production nationwide. Accordingly, it is important to investigate the effect of environmental factor, total number and age of ducks on egg production in Central Java. Specifically, this study aimed to observe the effect of region on egg production and feed conversion ratio (FCR) and the total livestock (chicken), age (month), stocking density (ducks/m2), internal housing temperature (oC), and humidity (%) on Hen day production/HDP. A survey engaging purposive random sampling was conducted on the population of duck farming in Central Java especially Tegal, Pemalang and Brebes districts. The data were subjected to General Linear Model (GLM) and a regression-correlation analysis using an SPSS program. The result showed that region significantly  affected hen day production (HDP) but did not affect FCR. Housing temperature affected HDP by 14.9% and the higher the temperature, the lower the HDP. Duck age affected HDP by 11.7%, and the older the ducks the lower the egg production. Duck population, stocking density and humidity did not significantly affect egg production. Conclusively, duck egg production (HDP) is significantly affected by temperature inside the housing and the duck age.

The purpose of this research was to determine the influence of honey supplementation in lactate ringer-egg yolk extender with 0.025% sodium dodecyl sulfate and 2% vitamin E addition (LREYSE) on the quality of Pelung chicken spermatozoa preserved at 5° C for 72 hours.Semen was collected from three Pelung chickens once per day over a course of three days using the dorsal-abdominal massage method. Semen was divided into 5 treatment groups of honey supplementation that are 0% as control (LREYSEH0), 1% (LREYSEH1), 2% (LREYSEH2), 3% (LREYSEH3), and 4% (LREYSEH4). This liquid semen was observed for sperm motility and viability every 12 hours. Complete random designrepeated measurement with 4 replications was used in this study. The results showed the motility and viability of spermatozoa in LREYSEextender with 2% honey supplementation (61.25±1.25% and 71.50±0.74%) was significantly higher (P0.05) than other treatments that are 0% (51.25±1.25% and 61.88±1.36%), 1% (52.50±1.44% and 63.25±1.38%), 3% (51.25±1.25% and 61.63±1.48%), and 4% (50.00±2.04% and 60.63±2.29%) of honey supplementation in extender at 36 hours of storage until the end of the observation at 72 hours of incubation. According to the results of this study, it can be concluded that the 2% honey supplementation in extender is the best treatment to maintain sperm motility and viability for 72 hours of storage.

The aim of this research was to investigate the influence of duck variants and addition of vitamins into muscovy duck semen on fertility and fertile period of duck eggs after artificial insemination. Semen was collected from five muscovy ducks and divided into 3 treatment groups: without vitamin supplementation (A0), supplementation of 400 μg/mL vitamin C (A1), and supplementation of 80 μg/ml vitamin E (A2). Each semen was inseminated into female ducks of Magelang (B1) and Mojosari (B2) variants. Complete Random Design was used with 3x2 factorial. The results showed that vitamins and duck variants had no significant interaction (P0.05) with fertility and fertile period. The duck variant had no effect (P0.05) on fertility and fertile period, while the addition of vitamins significantly affected (P0.01) the fertility and fertile period. The addition of 400 μg/mL vitamin C increased fertility by 22.28±0.20% but reduced the fertile period by 7.8±3.5 days, whereas 80 μg/mL of vitamin E increased fertility by 11.57±2.47% but reduced fertile period by 12.3±0.9 days. It can be concluded that the addition of 400 μg/mL of vitamin C and 80 μg/mL of vitamin E in Muscovy duck semen increased fertility but shortened fertile period of duck eggs after artificial insemination.

The objective of this experiment was to determine the addition of sodium dodecyl sulfate (SDS) to Ringer Lactate-Egg Yolk (RL-EY) extender on pelung chicken semen preservation. Semen was collected three times a week from three pelung chickens. Collected semen was evaluated macroscopically and microscopically. Only ejaculates of at least 70% sperm motility was then divided into three equal microtubes. Each of them diluted with RL-EY with ratio 90%:10%, then added with 0.00, 0.025, and 0.05% SDS respectively. The liquid semen then stored at 5 °C for 72 hours. Sperm motility and viability were observed every 12 hours. The addition of 0.025% SDS showed higher spermatozoa motility and viability (72.08±1.44% and 80.82±1.30%) which were significantly higher (P<0.05) than 0.00 and 0.05% SDS addition at 24 hours of storage. There was no differences on the spermatozoa motility and viability between 0.00 and 0.05% SDS addition. The decrease of spermatozoa motility and viability was observed in 0.025% SDS addition (4.17±0.56% and 4.65±0.59%, respectively) that significantly lower compared to 0% and 0.05% SDS addition at 24 hours of storage. In conclusion, 0.025% SDS addition in a Ringer Lactate -Egg Yolk extender maintained pelung spermatozoa motility and viability better than 0.00 and 5% SDS addition.