Abstract. Mushawwir A, Arifin J, Darwis D, Puspitasari T, Pengerteni DS, Nuryanthi N, Perman R. 2020. Liver metabolic activities of Pasundan cattle induced by irradiated chitosan. Biodiversitas 21: 5571-5578. A total of one hundred and twenty-five, 2-3 year old male Pasundan cattle were used as livestock samples during the three months of this research. They were selected from the local cattle breeding and development center in Ciamis. The animal samples were randomly allocated to 5 treatment groups. One group served as the control, or without irradiated chitosan, while the others were used as treatment in varying levels. Each treatment group involved five replicates with 25 Pasundan bulls per treatment i.e five Pasundan bulls per replication. Each group was provided with the following rations: C0 = Control group, without IC (0 ppm IC); C1 = 350 ppm Irradiated Chitosan (IC); C2 = 400 ppm IC; C3 = 450 ppm IC; and C4 = 500 ppm IC. Irradiated chitosan was obtained through the following steps: extraction, deacetylation, and irradiation of chitin using gamma rays. Five mL of blood samples were collected from each bull at the beginning of each month of this experiment, which totaled three months. The blood samples were sucked from the tail/coccygeal vein using a sterilized syringe and vacuum tube containing K3EDTA. The plasma was used to determine the concentration of parameters related to liver metabolism through an automatic biochemical analyzer Kenza 240TX model from Biolabo, using a commercial kit. Each procedure was followed based on the Biolabo kit (Franch) and Randox kit (UK). This study showed that IC reduces the activity of glycogenolysis and glycolysis, but is accompanied by improvements in the biochemical conditions of liver cells. This is a favorable condition for the metabolism of Pasundan bulls in order to enhance their growth and reproduction.

This experiment explores the effect and optimal levels of irradiated Chitosan (IrC) in the diet on lipogenesis and its effect on the blood lipid profile of the Sentul Chickens starter phase. The IrC was generated from shrimp waste chitin, and in addition to being a feed supplement, it has the potential to reduce environmental pollution. Furthermore, Sentul chickens were 100 samples, reared from 0-8 weeks, and the observed variables included triglyceride, cholesterol, and blood Non-Esterified Fatty Acid (NEFA). A completely randomized design (CRD) experimental method was used with four treatments and five replications. The treatments were K0 = basal diet without IrC, K1 = 300 ppm IrC in basal diet, K2 = 350 ppm IrC in basal diet, K3 = 400 ppm IrC in the basal diet. Meanwhile, the samples used for analysis were 40 individuals from the research object based on the average body weight of the population. The results showed that the provision of rations containing IrC (K1) 300 ppm, (K2) 350 ppm, and (K3) 400 ppm had a significant effect (P<0.05) on triglyceride, cholesterol, and NEFA profile in Sentul Chickens blood of the starter phase.

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The research aims to examine the effect of irradiated chitosan (ICh) on enzyme levels (SGPT, SGOT, and Gamma Transpeptidase) in the starter phase of Sentul chickens and its physiological conditions, as a result of fungal contamination from the environment (specifically from feed ingredients). This was conducted in the Poultry Production Laboratory, and the sample analysis was performed at the Lab of Animal Physiology and Biochemistry, Padjadjaran University. The samples consisted of 100 Sentul chickens reared from 0-8 weeks, and the method used was a completely randomized design (CRD) with 4 treatments and 5 replications. The treatments were K0 = basal diet (BD) without ICh, K1 = BD + 300 ppm ICh, K2 = BD + 350 ppm ICh, K3 = BD + 400 ppm ICh. The results showed that ICh was significantly different (P < 0.05) on SGPT, SGOT, and Gamma Transpeptidase levels. Therefore, it was concluded that the administration of 400 ppm ICh in the feed effectively enhanced the liver status of Sentul chickens in the starter phase.