A preliminary study was conducted to identify the effect of Spent Mushroom Substrate (SMS), a biomass from mushroom cultivation, towards catfish growth and intake performance when developed into fish feed. The fish experiment on catfish was conducted up to 12 weeks and analyzed on the weight, length size, survival rate and digestibility for the three types of SMS based feed in pellet form and a commercial pellet that applied for ten catfish each. Among the SMS based fish feed, Ganoderma obtained the highest weight of 32.46 g followed by White (30.71 g) and Abalone (17.74 g). For the length size, Ganoderma, White and Abalone SMS based feed had achieved in average 18.05 cm, 17.12 cm and 14.85 cm respectively. Commercial feed still manage to obtain the highest weight and length size as 39.33 g and 20.83 cm respectively. The survival rate was found 30 % higher in SMS based feed compared to commercial feed. The digestibility study of dry matter for Ganoderma, White and Abalone SMS based feed were found 77.34 %, 78.20 %, 77.76 % respectively whereas for commercial pellet was 60.92 % indicates that SMS based feed was consumed and digested better than commercial feed.
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