Objective: Human immunodeficiency virus (HIV) targets the immune system and weakens immune surveillance and defenses against infections,leading to acute immunodeficiency syndrome. Recent trends in drug discovery from natural sources emphasize investigations of compounds frommarine ecosystems.Methods: In this study, we compiled a database of chemical compounds from echinoderms and virtually screened for those that inhibit HIV-1 reversetranscriptase (RT). The database was generated from literature searches. Virtual screening analyses for inhibitors of HIV-1 RT were then performedusing AutoDock software.Results: Based on screening results, the top thirteen ranked compounds were nobilisidenol B, Ech_005, 17-deoxyholothurinogenin,22,25-oxidoholothurinogenin, Ech_022, Ech_026, Ech_021, nobilisidenol A, Ech_025, 5α-cholest-8(14)-ene-3ß,7α-diol, astropecten A, Ech_004, andphrygiasterol.Conclusion: The present in silico screening analyses of compounds from marine ecosystems can be used to identify candidate compounds with highpotential as drugs for the treatment of refractory HIV infections.