No abstract

Aspergillus awamori KT-11 is a proven potential mold produces the amylase enzyme complex, particularly amylase and glucosidase. These enzymes hydrolyze the starch being sugar. Food and beverage industry began extensive use of liquid sugar because it has several advantages. Liquid sugar is raw materials of starch in nature Indonesia. This purpose of this research is to extract powder enzyme Aspergillus awamori KT-11 from wheat bran against 4 types of raw material-based local carbohydrate to produce natural sweetener (liquid sugar).The raw material used namely potato, breadfruit, yellow squash, and bananas. Enzyme activity produced from mold Aspergillus awamori KT-11 on wheat bran of 186,9835 U/ml, reducing sugar of 9.775 mg/ml and protein levels is 48.981 mg/ml. The sweetener from natural potato starch, breadfruit, yellow squash, and banana produces different percentages. The highest percentage of banana flour produced at 117.938 mg/ml (concentration of flour 20%, enzyme 15% and incubation time for 3 days ) and lowest on yellow squash (6.563 mg/ml). The result shows that TLC from potato flour, breadfruit and bananas are produced of glucose and lactose, is from flour yellow squash generated fructose.

Endo-β-1, 4-xylanases is an enzyme that depolymerize xylan, a major component of lignocelluloses. Lignoselulose is a great source of cheap carbohydrate and thus has been used over the past decade as a raw material for the production of high value products, such as enzymes. However, lignocelluloses are a highly recalcitrant material that is extremely difficult to depolymerize. By using proper pretreatment, lignocelluloses from agroindustrial biomass can replace conventional carbon sources in media preparation for enzyme production. In this study, we used three kinds of agroindustrial biomass, such as sorghum variety of Buleleng, sorghum variety of JP, rice straw LIPI GO1 and these biomasses were pretreated with acid to remove a portion of lignin for use as a carbon source to endoxylanase production. The production of xylanase Kitasatospora sp. under submerged fermentation was investigated with different carbon sources using agroindustrial biomass. Optimization steps included studies carbon source concentration and pH medium fermentation. The optimized condition of enzyme production was obtained using the sorghum variety Buleleng biomass at 2% concentration and pH medium is 9.0 with activity 3. 04 U/mL.