infections are increasing now a day due to various factors. The present study was carried out to study the speciation and antifungal susceptibility pattern of isolated from blood and body fluids. Blood and body fluids received in the mycology laboratory of Microbiology Department, AIMSR, over a period of six months were included. After microscopy, culture was done. The spp. were diagnosed on the basis of colony characteristics, Gram staining and Germ tube testing and identification by Automated Vitek 2 system. Antifungal susceptibility testing was done by using YST08 cards. A total of 735 samples received, 53 showed Candida spp. Maximum spp. isolated from blood 23 (43.39%), followed by pleural fluid 14 (26.4%). were 24(45.3%) followed by 11(20.75%), 9(16.98%) 7(13.20%). spp. were sensitive to Amphotericin B and resistant to Fluconazole.