The present study investigated the quality and oxidative enzyme activity of catfish Hetreoclarias hybrid semen fortified with cucumber fruit juice. The highest milt fertility rate of 75.56% was recorded in 10% CJ on day 3 compared with the least rate of 56.21% in control on day 1. The least hatchability rate of 57.56% was recorded on control day 1 compared with the highest value of 75.07% in 10% CJ on day 2. The highest milt survival rate of 99.07% was recorded among the control group on day 3 compared with the least survival rate of 87.48% recorded on day 1 of 20% CJ. There was a significant difference (P< 0.05) between the CAT control and treatments of CJ. The lowest value of 0.29 μmolemm-1 mg protein-1 was recorded in control on days 1, 2 and 4 compared with the highest value of 0.34μmolemm-1 mg protein-1 in 15% CJ on day 4. There was no significant difference in SOD activity between the control and CJ. Recorded values ranged between 0,026-0.165 Umole-1mgprotein-1 in 15% on day 2 and 10% on day 1 respectively. There was no significant difference in LPO activity between the control and CJ. However, a range of 0.022-0.135 mMoleTbarsmm-1 protein-1 was recorded on day 1in 10% and day 4 in 15% respectively. 20% and 10% Cucumber fruit juice showed greater values in fertility rate than control at respective storage periods of 3 days in Hetreoclarias hybridfish semen, and could in combination with DMSO improve its cryopreservation at short periods.