Introduction and Aim: Globally, cardiovascular diseases are now the leading cause of death. A plant that has anti-inflammatory and antioxidant effects is Andrographis paniculata Nees.  The aim of the study was to determine whether A. paniculata Nees extract could increase Malondialdehyde (MDA) concentrations in the serum of rats with hypercholesterolemia.   Materials and Methods: The study used only a post-test control group with Thirty-six Wistar rats. The control group (K1) was not given both A. paniculata Nees and a high cholesterol diet, group 2 (K2) was given atorvastatin 5 mg/kg BW (positive control) and groups 4,5 and 6 were given A. paniculata Nees, respectively, 400, 800 mg/kg BW, following a high cholesterol diet. The concentration of MDA was checked on the 36th day using the TBARS method.   Results: Between treatment groups 1, 2, and 3, all three had a significant drop in total cholesterol levels after being administered the A. paniculata Nees extract for two weeks, however, the differences were not statistically significant (p=0.868).   Conclusion: The results of the present study showed no effect of A. paniculata Nees extract on the levels of malondialdehyde (MDA) in male Wistar rats with a hypercholesterolemia model.