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Aquarium fish industry has become a capacity for job creation and income generation. Aquarium fish uses for decoration and creating tranquility in the environment and beautification in public places. This study was conducted to investigate genetic variation between different populations of various varieties of guppy ornamental aquarium fish using inter simple sequence repeat (ISSR) markers in Iran for the first time. DNA was extracted from twelve strains. Polymerase chain reaction was performed using seven ISSR primers. GenAlex 6.501 and XLSTAT software was used to analyze data. Results of analysis of molecular variance showed that 54% of observed polymorphism belongs to variance among populations and 46% corresponds to diversity between populations. The highest and the lowest polymorphism percentage belonged to (GAG)5GC (90%) and (AG)8C (41%) markers, respectively. Percentage of polymorphic loci ranges from 32.31% to 86.15%. The results of phylogenetic tree showed that this tree was divided into 2 major clusters. Cluster I included only 2 populations and cluster II contained other 10 studied populations. It is suggested that this marker system was found to be efficient in discriminating each genotype at the molecular level and can be used for genetic diversity analysis for aquarium guppy fish because a greater understanding of the potential of the species is necessary for supporting long-term genetic improvement.

It was made the fi sh-biological assessment of yearlings carp hybrids obtained as a result of crossings of the Nivkovsky plant line of the small-scaled internal breed type of the Ukrainian Ramov breed and the Nivkovsky internal breed type of the Ukrainian scaly breed. The studies were carried out on the basis of the Institute of Fisheriesdepartment of the NAAS, Ukraine "Nivki", TOV "Skvirapleribribosp" and the laboratory of the Department of Production and Processing of Fish Products of the Belotserkovsky NAU according to practical standard in fi sh farming. As a control group, there were used carps of the Nivkovsky internal breed of Ukrainian scaly breed, with a total planting density of 30 thousand specimens. / ha. The research ponds were stocked with three-day larvae in early June. According to the content of the main cations and anions, nutrients and organic matter, the water of the research reservoirs of the Squirapleribribosp Ltd. for the period of the experiment corresponded to the existing standards. The quantitative and qualitative values of the indicators of the natural forage base were at the level of requirements for intensive rearing of young carp in pond farms. The growth rate of yearlings of the control group until the end of July was slightly higher than the studied group of carps hybrids by the average population values of the body mass index. The average body weight of yearlings in the control pond with the origin of LF * LF was at the moment of the experiment 7.3 ± 0.5 g, for carps of the origin of LF * NMF - 6.8 ± 0.6 g. At the end of September, the value for this indicator was at the level of 25.5 ± 1.6 g and 27.0 ± 2.0 g, respectively, for the control and the group of crossbreeds. The indices of heterosis in yearlings of carps hybrids had positive values accordance with all fi sh and biological indicators relative to their parental forms: by average weight - 106.7%, output - 111.5%, and fi sh production - 118.1%. It was established that a mixture of carps of the NCHK * NMK group have high cold resistance and winter hardiness, which are characteristic of the parent form of the Nivka lowscale carps. Key words: carp, breeding, Nivkovsky scaly, Nivkovsky scaly, yearlings, heterosis index, fi sh-biological indicators, winter hardiness.

The Ros River is polluted with heavy metals, petroleumproducts and organic matter due to the activities of variousindustries, including agriculture (washing away pesticides and fertilizers), food, light and petrochemical industries, etc.Intake of drinking water in Bila Tserkva district is carried outfrom the river Ros, so the aim of the work was to systematizethe results of research over the past 10 years and identify thedynamics of changes in the ecological state of the river.Object of research: Ros River within Bila Tserkva district of Kyiv region.Subject of research: negative environmental factors,water quality indicators. The study of the state of the question was carried out by methods of analysis and synthesis.Water quality assessment was conducted in the Ros Riveron a monthly basis. Initial data (monthly results) are averaged by seasons: spring (March-May, 2010-2020), summer(June-August, 2010-2020), autumn (September-November,2010-2020), winter (December of the previous year - January, February of the current year, 2010/2011-2020/2021).Among the studied indicators there are ammonium salt, nitrates, nitrites, chemical oxygen demand (COD), biochemicaloxygen demand (BOD5), phosphates, total iron, manganese.Seasonal concentrations throughout the study period for ammonium salt ranged from 0.11 to 2.17 mg/dm3, nitrites – from0.01 to 0.82 mg/dm3, nitrates did not exceed 40.0 mg/dm3,COD– in the range from 15.9 to 61.1 mg/dm3, BOD5– in therange from 1.5 to 8.3 mg/dm3, phosphates – from 0.05 to 0.49mg/dm3. Excess concentration of total iron and manganesewere observed in 59% and 29% of cases, respectively. Thatis, there is a tendency to deterioration water quality by somecomponents; the main reason for this is anthropogenic activity, namely the inconsistency of water treatment equipmentof economic entities with modern environmental standardsand the presence of unidentified sources of discharges intothe Ros River basin. Key words: Rosriver, hydrochemical indicators, ecological condition, seasonal values, MPC.