The purpose of these studies was to determine status of metabolic processes in clinically healthy horses of sport breeds in spring period. Blood samples for biochemical studies were collected from 12 clinically healthy 7–9 month-old stallions of Ukrainian horse breed at Dnipropetrovsk region equestrian club. Protein (albumin, globulin, urea and creatinine) and mineral (common calcium and inorganic phosphorus) metabolic statuses, level of glucose, vitamins A and E and acid, as well as activity of hepatospecific enzymes (ALT, AST and AP) were determined using common techniques. It has been found that common protein level was within the limits of physiologic norm, although the level of albumins was decreased at the average rate of 12.0%, and the level of β- and γ-globulins was increased at the average rates of 5.2 and 11.3% respectively. AST activity was decreased at the rate of 38.0% regarding to physiological norm. Thereby, urea and creatinine concentrations were within the referent levels. Hyperglycaemia was observed in 50.0% of tested animals with maximal excess at the rate of 44.0%. Also, decreasing of common calcium and inorganic phosphorus levels was determined at the rate of 16.0 and 58.6%, vitamins А and Е — at the rate of 64.0 and 48,6% respectively, in comparison to lower level of physiological norm. The average index of acid capacity reached maximal referent levels. At the same time, it was increased in 33.3% of animals. Therefore, detected changes in biochemical indices in horse blood evidence that various metabolic disorders progress in clinically healthy stallions at spring and may furtherly lead to the appearance of metabolic syndrome

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The article highlights the results of studying the effect of a new probiotic nanometal globulin drug (PNMGD) on biomarkers of innate immunity of newborn calves. The experiment was performed on two groups of calves. Animals of the experimental group from the 2nd day of life were fed the drug for 5 days at a dose of 0.25 g/kg of body weight with milk, calves of the control group received milk without its addition. Before the experiment, and on the 10th, 20th, 35th day of the experiment, blood was taken from calves for clinical and biochemical studies. The obtained results show that the use of PNMGD causes an increase in the natural resistance of calves. This is indicated by an increase in the expression of such markers of innate immunity as globulins, circulating immune complexes and nitrogen metabolites by an average of 17–25%, as well as inhibition of seromucoid synthesis by 16.9%. Signs of anti-stress effect of the drug on the calves in the early postnatal period have been found. The positive effect of PNMGD on the state of innate immunity of calves can be regarded as one of the factors increasing the average daily weight gain by 32.2% in the first 36 days of life

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The purpose of the study was to determine the focus and the intensity of the effect of the developed feed supplement based on the silkworm pupae on the nonspecific humoral immunity of poultry. The research was carried out on one-day-old broiler chickens, which were kept in standard vivarium conditions. The birds of first group were given the supplement in the morning, mixing with compound feed at the rate of 3.0 ml/kg of live weight. The second group was control. On the 20th, 27th, 34th and 48th days, 5 birds from each group were euthanized and blood was collected for clinical and biochemical studies. To determine the indicators of non-specific humoral immunity of birds after receiving feed supplement based on silkworm pupae in the blood serum of birds, the level of total protein, albumins, globulins, the concentration of circulating immune complexes of average molecular weight and seromucoids were determined by generally accepted methods. As a result of the use of a feed supplement based on silkworm pupae, there was determined an increase in the level of hemoglobin up to 16.0% and a slight activation of erythropoiesis, an increase in the level of humoral immunity, as evidenced by an increase in globulins up to 12.2% and circulating immune complexes up to 30.7%, and also inhibition of seromucoid synthesis by 15.4%. Thus, it has been concluded that the use of a feed supplement based on silkworm pupae allows to increase the level of non-specific humoral immunity of chickens, as evidenced by increased expression of its markers (globulins, circulating immune complexes), as well as inhibition of seromucoid synthesis. The revealed positive effect of the feed supplement based on silkworm pupae on the state of natural resistance can be considered as one of the factors of increasing the average daily weight gain of chickens by 39.0% in the first 7 days of life