Abstract:In this research work, melon oil was used as feedstock for methyl ester production. The research was aimed at optimizing the reaction conditions for methyl ester yield from the oil. Response surface methodology (RSM), based on a ve level, four variable central composite designs (CCD) was used to optimize and statistically analyze the interaction e ect of the process parameter during the biodiesel production processes. A total of 30 experiments were conducted to study the e ect of methanol to oil molar ratio, catalyst weight, temperature and reaction time. The optimal yield of biodiesel from melon oil was found to be 94.9% under the following reaction conditions: catalyst weight -0.8%, methanol to oil molar ratio -6:1, temperature -55• C and reaction time of 60mins.The quality of methyl ester produced at these conditions was within the American Society for Testing and Materials (ASTM D6751) speci cation.