This study investigated the prevalence and antibiotics susceptibility of Enterococcus spp. isolated from patients and some selected hospital environment in Abuja, Nigeria. The samples included clinical and environmental. The clinical samples included stool, urine and wound swabs while the environmental samples included swabs samples taken from the health care givers hands, floor, beds, door handle, BP cuff, stethoscope, sink, toilet seats. The samples were cultured on bile aesculinazide agar and the isolates were identified with microgen test kit. The enterococcal strains isolated include Enterococcus faecalis, Enterococcus faecium, Enterococcus mundtii, Enterococcus gallinarum, Enterococcus casseliflavus, Enterococcus dispar, Enterococcoushirae and Enterococcus avium. The susceptibility testing was done with vancomycin, teicoplanin, gentamicin, streptomycin, linezolid, ampicillin, ciprofloxacin, chloramphenicol, doxycycline, nitrofurantoin, erythromycin and rifampin. More than 50% of the isolates were resistant to erythromycin, rifampin and doxycycline. E-test M.I.C confirmed 12 out of 34 strains to be intermediately resistant to vancomycin. Enterococcus faeciumand Enterococcus mundtii exhibited more resistance than Enterococcus faecalis.