In the industrial pig farm for 26 thousand heads, the analysis of the influence of a forage factor on bacteria carriers of a uterine pig population in connection with mass morbidity of dairy piglets on anaerobic enterotoxemia is carried out. Actinobacillus pleuropneumonia, Pasteurella multocida, Clostridium perfringens, Neisseria spp., Candida albicans, Aspergillus niger which are able to form biofilms, were isolated from five samples of “SK-1” compound feed for pregnant sows and from the blood of animals (n=20) fed with this compound feed. The structural basis of the most stable biofilms in vitro were the aerobic fungi Aspergillus niger and Candida albicans. Biofilm-forming variants of these bacteria showed multidrug resistance to 30 antimicrobial drugs (synthetic penicillins, cephalosporins, fluoroquinolones, aminoglycosides, tetracyclines, combination drugs). Isolates of associative microflora isolated from the blood of sows were pathogenic for 30% of laboratory mice. It was found that probiotic agent No1 (composition based on Bischofite with probiotics) showed the universal bactericidal activity against the bacteria Actinobacillus pleuropneumonia, Pasteurella multocida, Clostridium perfringens, Neisseria spp.

No abstract

The novel coronavirus SARS-CoV-2 that causes COVID-19 disease, was mainly described as a something extraordinary in media discourse. Its “unusual,” almost “mystical” properties have been constantly invented by world media sources since January 2020 thus far. Critical comparison of the real epidemiological, physical, chemical and biological properties of SARS-CoV-2 with most common causative agents of coronavirus family demonstrates that principles and techniques of laboratory diagnostics and prophylaxis of SARS-CoV-2 should be built only on the basis of evidence-based medicine, not on imaginary properties of the virus that were created with ideological purposes.

B.T. Stegniydocteur en sciences vétérinaires, académicien de l'Académie nationale des sciences d'Ukraine, directeur de l'Institut de médecine vétérinaire expérimentale et clinique (Kharkiv)

Research results and discussion. All vaccinated adult mouse (n=12) at contrast to unvaccinated (n=8) were alive after its inoculation with broth culture of Mannheimia haemolytica in dose 0.5 cm 3 (10 5 BC 50) during 1 month of trials. Level of vaccinated mouse seroconversion on PRRS, PCV-2 and bacterial antigens was 0; 1/2.30+0.11 and 1/351.00+5.73 (P<0.01), in correspondence. All vaccinated pigs (n=5) after first injection had a levels of seroconversion on PRRS, PCV-2 and bacterial antigens (3 week after vaccination) 1/8.80+2.05; 1/22.50+7.73 and 1/51.00+6.88 (P<0.05), in correspondence. After revaccination (3 week after first injection) pigs had a levels of seroconversion on PRRS, PCV-2 and bacterial antigens (3 week after second injection) 1/17.40+0.78; 1/34.23+0.73 and 1/121.11+2.44 (P<0.05), in correspondence. Conclusions. Modified vaccine "ReproSuiVac-IECVM" are ready to clinical trials against the concurrent ASF-hemolytic pasteurellosis infections that promote of blind spreading of ASF agent in the quarantine areas of ASF outbreaks (promote of its attribution) in Ukraine.