Strawberry is the main berry crop with a fast-growing global market. With the mass production of high-quality planting material, the role of biotechnological methods of reproduction increases. The aim of the study was to study the impact of various auxins on the rhizogenesis of popular commercial varieties of domestic and foreign garden strawberries in the system of production of healthy planting material. The research was carried out in the laboratory of biotechnology of the GNU VNIISPK according to generally accepted methods. The objects of the study were varieties of domestic and foreign garden strawberries: Bereginya, Urozhainaya CGL, Tsaritsa, Asia, Darselekt, Kimberly, Korona, Marmolada, Frida, Honeoye. The conducted studies have revealed a high rooting ability of the studied varieties. After 6 weeks, 100% rooting of strawberry varieties was noted on all inductors of root formation. The optimal types, concentrations and combination of rhizogenesis regulators providing intensive root formation in plants of the studied varieties have been determined. In the microarrays of Asia and Honeoye varieties, the largest number of roots was observed when using IAA at a concentration of 0.5 mg/l (6.8 pcs. / explant). Kimberly and Tsaritsa plants more actively formed a voluminous root system with the addition of BMI – 0.5 mg/l (5.6 and 5.8 pcs/exp. respectively). An increase in the concentration of IBA to 1.0 mg/l did not contribute to an increase in the number of roots in the studied varieties. An increase in the concentration of IBA to 1.0 mg/l did not contribute to an increase in the number of roots in the studied varieties. The joint introduction into the nutrient medium of auxins of IBA and IAA at a concentration of 0.5 mg /l can be recommended for rooting micro-rosettes of varieties Asia, Bereginya, Darselekt, Marmolada, Urozhainaya CGL. Their synergistic effect influenced the formation of more longer roots.