No abstract

The article presents the results of determining the level of expression of genes encoding GABA receptor subunits GABRA1, GABRB2, and GABRG2 in the hippocampus of rats 24 hours after a single intramuscular administration of the antagonist of the chloro-ion channel of GABA receptor of kloflubicin in a dose of LD25, LD40, LD50, and LD75. It is revealed that kloflubicin has no influence on the relative level of GABRG2 gene expression. At the same time, the relative level of GABRA1 gene expression increases 7,5, 7,0, and 5,0 times after administration of kloflubicin in a dose of LD40, LD50, and LD75, respectively. The relative level of GABRB2 gene expression also increases 3,6 and 2,6 times after administration of kloflubicin in a dose of LD50 and LD75, respectively. It is assumed that increase in the level of gene expression of GABRA1 and GABRB2 in rats after administration of kloflubicin in doses above LD25 is a compensatory reaction of the body to the effect of physiologically active substance, and subunits encoded by these genes α1 and β2, respectively, are included in the mechanism of convulsive effect.

Four polymorphic GABRA2 gene variations: rs105733011, rs8168342, rs198286814, rs198837638 that can influence the formation of different biological effects of the organism when exposed to ethanol have been investigated. For rs105733011 polymorphism the frequency of occurrence of the CT genotype was found to be significantly higher (p < 0,05) among animals with «severe intoxication» – 37,0% than with «mild intoxication» – 14,0%. For rs198286814 polymorphism the tendency to the most frequent occurrence of the AG genotype in the group of animals with «severe intoxication» was established. Significant differences in the distribution of occurrence frequencies of the GG/AG genotypes in the studied groups for polymorphic loci rs8168342 and rs198837638 were not revealed. It was concluded that the rs105733011 polymorphism can be one of the genetic markers allowing to predict the degree of inhibitory action of ethanol in acute alcohol intoxication.

No abstract

Infant colic is a common cause of parental anxiety and, as a result, help-seeking behaviour. However, the relationship between breastfeeding and colic is currently not fully understood. The article presents data from various studies of the relationship between breastfeeding and colic. On the one hand, the severity of infant colic may lead to the early cessation of exclusive breastfeeding due to the mother’s fear that the baby’s crying is the result of the ineffectiveness of breastfeeding and the unreasonable transition to supplemental feeding. On the other hand, many authors have identified the protective effect of breastfeeding in relation to infant colic a less pronounced degree of infant colic is reported in the group of children, who are breastfed as compared to the children, who are formula-fed. Therefore, the task of the paediatrician is to provide competent counselling for the anxious parents of an infant with colic using evidence-based medicine (EBM) databases, which will help preserve the mother’s breastfeeding. In most cases, due to competent counselling of the parents, the severity of infant colic may be reduced by improving the quality of breastfeeding (ensuring proper breast grabbing to reduce aerophagy, increasing the proportion of consumption of the so-called «back» milk), reducing anxiety of the parents and increasing the perception of breastfeeding self-efficacy levels by mothers. This may increase the duration of breastfeeding of the infants with infant colic.