Neuroengineering methods can be effectively used in the design of new approaches to treat central nervous system and brain injury caused by neurotrauma, ischemia, or neurodegenerative disorders. During the last decade, significant results were achieved in the field of implant (scaffold) development using various biocompatible and biodegradable materials carrying neuronal cells for implantation into the injury site of the brain to repair its function. Neurons derived from animal or human induced pluripotent stem (iPS) cells are expected to be an ideal cell source, and induction methods for specific cell types have been actively studied to improve efficacy and specificity. A critical goal of neuro-regeneration is structural and functional restoration of the injury site. The target treatment area has heterogeneous and complex network topology with various types of cells that need to be restored with similar neuronal network structure to recover correct functionality. However, current scaffold-based technology for brain implants operates with homogeneous neuronal cell distribution, which limits recovery in the damaged area of the brain and prevents a return to fully functional biological tissue. In this study, we present a neuroengineering concept for designing a neural circuit with a pre-defined unidirectional network architecture that provides a balance of excitation/inhibition in the scaffold to form tissue similar to that in the injured area using various types of iPS cells. Such tissue will mimic the surrounding niche in the injured site and will morphologically and topologically integrate into the brain, recovering lost function.

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The lack of a clear structural vision of the national model of socially-oriented economy leads to significant social disparities and distortions in society. Due to the undeveloped transparent concept of social policy, the development of social processes becomes unmanageable. The key issues facing most countries, both in the short and long term, are to build an effective system of social protection and social security that can solve urgent and permanent social problems. The need for rethinking the concept of national social policy, defining a clear goal, objectives, mechanisms and stages of its implementation is most acute. This problem becomes especially relevant in light of the processes of instability of socio-economic development of the state and the military conflict in eastern Ukraine. The article substantiates the theoretical and methodological principles of the mechanism of formation and implementation of state policy in the field of employment in Ukraine; proposed directions for the development of the system of providing services in the field of employment aimed at preventing unemployment and increasing the competitiveness of citizens in the labor market; the tendencies of development of components of modern economy and society which cause transformation of structure of the employed citizens are allocated; the priorities and models of the state social policy and the directions of their changes in modern conditions are studied; generalized by systematizing the experience of functioning of models of social protection in order to identify opportunities for its application to the national economic system; the current crisis state of the national economy (in terms of deformation of the socio-economic subsystem, the risks of deepening social inequality) and the conditions of social protection are analyzed; an algorithm for improving the system of social services in the field of employment and the scientific concept of economic development in the modern transformation of the national economy on social security processes using the principle of inclusive social growth in order to form the main components of social protection.

The aim of the study was to develop a method for long-term non-invasive recording of the bioelectrical activity induced in isolated neuronal axons irradiated with short infrared (IR) pulses and to study the effect of radiation on the occurrence of action potentials in axons of a neuron culture in vitro . Materials and Methods. Hippocampal cells of mouse embryos (E18) were cultured in microfluidic chips made of polydimethylsiloxane and containing microchannels for axonal growth at a distance of up to 800 μm. We studied the electrophysiological activity of a neuronal culture induced by pulses of focused laser radiation in the IR range (1907 and 2095 nm). The electrophysiological activity of the neuronal culture was recorded using a multichannel recording system (Multi Channel Systems, Germany). Results. The developed microfluidic chip and the optical stimulation system combined with the multichannel registration system made it possible to non-invasively record the action potentials caused by pulsed IR radiation in isolated neuronal axons in vitro . The propagation of action potentials in axons was detected using extracellular microelectrodes when the cells were irradiated with a laser at a wavelength of 1907 nm with a radiation power of 0.2–0.5 W for pulses with a duration of 6 ms and 0.5 W for pulses with a duration of 10 ms. It was shown that the radiation power positively correlated with the occurrence rate of axonal response. Moreover, the probability of a response evoked by optical stimulation increased at short optical pulses. In addition, we found that more responses could be evoked by irradiating the neuronal cell culture itself rather than the axon-containing microchannels. Conclusion. The developed method makes it possible to isolate the axons growing from cultured neurons into a microfluidic chip, stimulate the neurons with infrared radiation, and non-invasively record the axonal spiking. The proposed approach allowed us to study the characteristics of neuronal responses in cell cultures over a long (weeks) period of time. The method can be used both in fundamental research into the brain signaling system and in the development of a non-invasive neuro-interface.

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