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An important element in ensuring the epizootic well-being of the poultry industry is disinfection. Modern poultry farming requires a large number of effective disinfectants. It is known that the resistance of microorganisms to the effects of disinfectants is based on a genotypic mechanism. The nature of the formation of resistance to disinfectants and antiseptics is different than antibiotics. With regard to disinfectants, resistance is formed more slowly and the proportion of resistant strains in the population of microorganisms may not be high for a long time. This is due to different mechanisms of formation of resistance to antibiotics and disinfectants, in the first case – plasmid mechanism, in the second – chromosomal. However, increasing the resistance to the active substance in disinfectants can be widespread, so it is necessary to periodically rotate disinfectants. The goal of the work – to investigate the parameters of acute toxicity of the disinfectant biocide “Zodizin”. The studies were conducted in the laboratory of Veterinary Pharmacy and the Vivarium of Sumy National Agrarian University. The drug “Zodizine” contains: polyhexamethyleneguanidine hydrochloride – 21.0 %, alkylldimethylbenzylammonium chloride – 3.0 %. For toxicological examination of the disinfectant, healthy white male rats and white female rats weighing 200 ± 10 g 1.5 years of age were used. In the study of acute toxicity of animals observed daily, noted the general condition of the animals, features of their behavior. Studies have found that the toxic effect of the disinfectant “Zodizin” clinically manifested almost equally in both males and females. The average lethal dose for the rat female was 1000.0 ± 35.0 mg/kg body weight, males 1033.0 ± 34.3 mg/kg. Therefore, according to the classification of substances by toxicity, the drug by intragastric administration can be attributed to low-toxic substances. Observations on animals revealed that 1–3 hours after oral administration of the drug in a subtoxic dose in laboratory animals, shortness of breath and inhibition of the central nervous system were noted. Most of them died during the first day. Subsequent observations of the surviving animals indicated that their motor response was suppressed over the next 24–72 hours. Conclusions and prospects for further research: 1. It was found that the average lethal dose of the drug “Zodizin” with oral administration to rats-females was 1000.0 ± 35.0 mg/kg body weight, males – 1033.0 ± 34.3 mg/kg. 2. Experimental studies have proved that the disinfectant “Zodizin” according to GOST 12.1.007-76, belongs to the IV class of danger, that is, to the low-dangerous compounds, and according to GOST 12.1.07 – to the III class of hazard of substances and can be used for disinfection premises where animals and poultry are kept. Further, the sporoсide and corrosion properties of the “Zoodizin” biocide will be studied.

The purpose of these studies was to analyse and compare the content of bromine in samples of chicken eggs, feed, and water from different regions of Ukraine in the dynamics of 2016 – 2020: with an increased risk of bromine in products (Kharkiv, Poltava, Dnipropetrovsk and Mykolaiv regions) and outside the risk zone (Volyn, Vinnytsia and Zaporizhzhia). Studies of bromine content in eggs, feed, and water were performed in the laboratory of toxicological monitoring of the National Scientific Center "Institute of Experimental and Clinical Veterinary Medicine" (Kharkiv) using X-ray fluorescence analysis. As a result of the conducted researches, the increase of the bromine content in chicken eggs in the dynamics of 2016 – 2020 was established: the bromine content increased regardless of the region of the poultry farm location. The highest bromine concentration in chicken eggs was found in Kharkiv, Dnipropetrovsk, Mykolaiv, and Zaporizhia regions. Bromine source in poultry products is the excessive intake of bromine in the poultry body with alimentary environmental factors (feed and water). Bromine content in feed for chickens increased in the research dynamics (from 35.1% in the Poltava region to 2.5 times in the Zaporizhzhia region). It exceeded the established EFSA (4.4% of the total) and the average in Ukraine (51.2% of the total number of samples). In addition, the average bromine content in feed from poultry farms of the studied regions of Ukraine correlated with the number of registered and approved bromine-containing pesticides. The average bromine concentration in water sources in the studied regions of Ukraine had no significant differences compared to the beginning of the study but exceeded the maximum allowable concentration by 21.7% in 2016, 34.8% in 2018 and 39.1% in 2020. The maximum bromine concentration was in water sources in Mykolayiv, Kharkiv, and Dnipropetrovsk regions.

The key to successful work of a modern poultry industry and the safety of its products for consumers is high-quality disinfection. Modern disinfectants used in poultry farms are different in composition, concentration of active substances, means of application and other properties. Most of them are represented by imported products. Their replacement in the domestic market with effective, safe and economical drugs is extremely relevant. It should be highly effective means, possessing a wide spectrum of virilicidal, bactericidal, sporocidal and fungicidal action. The goal of the work. The purpose of our research was to evaluate the effectiveness of the complex disinfection in the production conditions of the Bi-Dez and DezSan products. Materials and methods of research. Production testing of the disinfection of poultry facilities and equipment using the Bi-dez and DezSan schemes was carried out at one of the poultry farms of the Sumy region. At the first stage, the water supply system was treated by filling the piping line with a 0.1% solution of Bi-dez with an exposure of 1 hour. At the second stage, moisture was disinfected with 0.8% solution DezSan from the rate of 0.2-0.3 liters of solution per 1 m 2 , exposure time -2-3 hours. Then, in the room, the fine dispersed aerosol of this biocide was sprayed into a 10% solution (1 liter of preparation per 9 liters of water) -aerosol disinfection by fogging at a rate of 5 ml of solution per 1 m 3 of space, exposure time -at least 3 hours. Results of research and discussion. Evaluating the effectiveness of disinfection of the water supply system with the Bi-dez compound, found that the total microbial contamination of the water before treatment was 235 CFU/ml. In addition to other bacteria, it was isolated from the intestinal bacillus. After the rehabilitation, the microbial water pollution decreased to 4 CFU/ml. Sanitary-indicative microorganisms (E. coli, salmonella and staphylococci) were not detected in the system faults. The total amount of microorganisms in the air after disinfection with the drug DezSan decreased by 2.6 times (12 thousand mc/m 3 ) compared with the baseline bacterial background (29 thousand mc/m 3 ), and the intestinal rod after treatment from the selected in the poultry house air samples were not allocated. Conclusions and prospects for further research. Reducing the microbial background and the death of opportunistic microorganisms in the premises and the system of drinking poultry farms contribute to the veterinary well-being of poultry farms. In the future, it is planned to conduct a study of the drug DezSan, identified it irritating, skinresorptive, cumulative and other properties.