The aim: Evaluation of the influence of plant protection chemicals on the fatty acid composition of sunflower seeds. Materials and methods: Study of the effects of pesticides, study of the effects of pesticides on the fatty acid composition (lipid) of sunflower seed by gas-liquid chromatography was studied. Results: It was found that the content of oleic and linoleic unsaturated fatty acids did not differ significantly from the control. The content of linolenic and arachidonic acids was at the level of the control group. A similar pattern was observed in relation to the content of saturated fatty acids, in particular myristic, pentodecanoic, palmitic, margaric, stearic. The total content of fatty acids (unsaturated fatty acids and saturated fatty) selected under different seed protection schemes did not differ significantly from control. Conclusions: It is proved that the application of the investigated pesticides in various schemes of chemical protection of sunflower crops does not affect the nutritional and biological value of the seeds.

Introduction: The research of biological properties of enteroviruses associated with ischemic stroke (IS) allows us to identify their intratypic differences. The aim: to identify genetic markers of strains of enteroviruses associated with IS. Materials and methods: 11 strains of enteroviruses isolated from the serum of patients with IS were identified in the virus neutralization test. Genetic markers of isolated strains (Abent, marker S, marker rct40) were determined. Results: Eleven strains of enteroviruses were isolated from the serum of patients with IS. Eight viruses: Coxsackie B viruses (serotypes 2, 3, 4) and ECHO viruses (serotypes 6, 9, 27 (two strains), 29) were identified in these strains. Other three strains of enteroviruses were unidentified. Different combinations of genetic markers were found. Seven strains of enteroviruses (Coxsackie B2, B3, ECHO 6, ECHO 9, ECHO 27 (two strains) and one unidentified virus) had virulence markers: Abent–, rct40+ and S−. Three strains (Coxsackie B4, ECHO 29, one unidentified virus) had markers: Abent–, rct40+, S+. Another one unidentified virus had markers: Abent+, rct40+, S –. Conclusions: All 11 isolates of enteroviruses associated with IS had rct40+ marker, 10 of the 11 isolates had marker Abent– and 8 of 11 isolates had marker S–. The research of genetic markers allows to perform typic and intratypic differentiation of strains of enteroviruses associated with the IS.

The paper presents the features of the development of endothelial dysfunction in workers under lead exposure. The results of laboratory manifestations of endothelial dysfunction have been demonstrated on the base of the study of parameters of vascular molecule of adhesion vCaM-1, soluble molecule of intercellular adhesion Sіcam-1, endothelin-1 in the blood of workers who professionally contact with lead. The features of formation of endothelial dysfunction, depending on the content of lead in the blood were identified. The thresholds for the studied parameters of endothelial dysfunction which are associated with high risk of atherosclerosis in this category of workers were determined.

Lymphangioma is a benign lymphatic system tumor, often congenital, mainly affecting lymph nodes in various anatomic localizations. Postnatally mostly common lymphangioma manifestation occurs during the first year of life. Today it can be readily diagnosed prenatally. Diagnosis is based on non-invasive visualization, such as sonography and computed tomography. The objective: is to present clinical case of manifest fetal lymphangioma diagnosed during pregnancy. Fetal ultrasound (US) was performed at 28, 32, 34 weeks of gestation. As a result fetus was diagnosed with hypoechogenic mass in right axillary region, growing in dynamics gradually from 34×28 mm, 38×34 mm to 49×37 mm. Mass had clear even contours, hyperechogenic capsule with fine dispersed suspension content. Color doppler imaging showed avascular mass character. After delivery at 37–38 weeks of gestation (planned Cesarean section) at newborn examination we visualized asymmetric soft elastic consistency protrusion along right midclavicular and anterior axillary region. Described clinical case of fetal lymphangioma is a good illustration of dynamic prenatal and postnatal dynamic observation and its US interpretation.

The aim: To study the role of enteroviruses (EV) in the development of ischemic stroke and its outcome. Materials and methods: The main group (MG) included 72 patients with acute cerebrovascular disorders were examined using the National Institutes of Health Stroke Scale and Barthel Index. The comparison group (CG) included 35 patients without cerebrovascular disease. Viruses were isolated from patients’ sera and identified in neutralization test. EV genomes were detected in polymerase chain reaction (PCR). Serological diagnosis was performed by enzyme-linked immunosorbent assay. Results: EV genomes were more frequently detected in the patients’ sera in MG than in CG (23.6 ± 5.9% and 2.9 ± 2.8%, p <0.05). The greater level of neurological deficits was in patients with positive PCR test results comparatively with patients with negative PCR test results (11.76 ± 0.31 and 10.97 ± 0, 27, p = 0.040). The regression of neurological deficit during the treatment was a worse in patients with positive PCR test results and presence of specific IgG compared with patients with positive PCR test results and absence of specific IgG (11.2 ± 2.6% and 19.6 ± 2.4%, p = 0.031). Conclusions: The trigger role of EV in the development of IS is established. PCR is recommended for diagnosis of EV in patients with IS.