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Oncorhynchus keta has been coupled completely with 1-fluoro-2:4-dinitrobenzene and the spectrum of the resulting dinitrophenyl (DNP)-salmine measured in the range 235-420 m,u.2. The spectrum is similar to the spectrum of DNP-proline.3. Four-fifths of the N-terminal residues in the salmine are proline and the rest mainly arginine, serine and glycine.4. From the spectrum an average molecular weight of 3800 ± 10 % has been calculated for salmine, assuming all the molecules to be simple open-chain peptides. 5. A secondary slow reaction occurs with fluorodinitrobenzene, which is unexplained.6. A little N5-DNP-ornithine was found, which it is thought may be in the native protamine and not a degradation product.I wish to thank Mr D. A. Maguire for his continued valuable technical assistance and Dr J. A. Broben of the Commonwealth Serum Laboratories, Melbourne, for the gift of a sample of protamine from Salmo irideus.

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