Recent studies highlighted an emerging possibility of using Drosophila as a model system for investigating the mechanisms of human congenital muscular dystrophies, called dystroglycanopathies, resulting from the abnormal glycosylation of alpha-dystroglycan. Several of these diseases are associated with defects in O-mannosylation, one of the most prominent types of alpha-dystroglycan glycosylation mediated by two protein O-mannosyltransferases. Drosophila appears to possess homologs of all essential components of the mammalian dystroglycan-mediated pathway; however, the glycosylation of Drosophila Dystroglycan (DG) has not yet been explored. In this study, we characterized the glycosylation of Drosophila DG using a combination of glycosidase treatments, lectin blots, trypsin digestion, and mass spectrometry analyses. Our results demonstrated that DG extracellular domain is O-mannosylated in vivo. We found that the concurrent in vivo activity of the two Drosophila protein O-mannosyltransferases, Rotated Abdomen and Twisted, is required for O-mannosylation of DG. While our experiments unambiguously determined some O-mannose sites far outside of the mucin-type domain of DG, they also provided evidence that DG bears a significant amount of O-mannosylation within its central region including the mucin-type domain, and that O-mannose can compete with O-GalNAc glycosylation of DG. We found that Rotated Abdomen and Twisted could potentiate in vivo the dominant-negative effect of DG extracellular domain expression on crossvein development, which suggests that O-mannosylation can modulate the ligand-binding activity of DG. Taken together these results demonstrated that O-mannosylation of Dystroglycan is an evolutionarily ancient mechanism conserved between Drosophila and humans, suggesting that Drosophila can be a suitable model system for studying molecular and genetic mechanisms underlying human dystroglycanopathies.

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Abstract:The paper discusses our studies of oil pollution in the Black and Caspian Seas. The research was based on a multi-sensor approach on satellite survey data. A combined analysis of oil film signatures in satellite synthetic aperture radar (SAR) and optical imagery was performed. Maps of oil spills detected in satellite imagery of the whole aquatic area of the Black Sea and the Middle and the Southern Caspian Sea are created. Areas of the heaviest pollution are outlined. It is shown that the main types of sea surface oil pollution are ship discharges and natural marine hydrocarbon seepages. For each type of pollution and each sea, regions of regular pollution occurrence were determined, polluted areas were estimated, and specific manifestation features were revealed.Long-term observations demonstrate that in recent years, illegal wastewater discharges into the Black Sea have become very common, which raises serious environmental issues. Manifestations of seabed hydrocarbon seepages were also detected in the Black Sea, primarily in its eastern part. The patterns of surface oil pollution of the Caspian Sea differ considerably from those observed in the Black Sea. They are largely determined by presence of big seabed oil and gas deposits. The dependence of surface oil SAR signatures on wind/wave conditions is discussed. The impact of dynamic and circulation processes on oil films drift and spread is investigated. A large amount of the data available allowed us to make some generalizations and obtain statistically significant results on spatial and temporal variability of various surface film manifestations. The examples and numerical data we provide on ship spills and seabed seepages reflect the influence of the pollution on the sea environment.

Abstract:The paper discusses the results of a study of short-period internal waves (IWs) in the Black and Caspian Seas from their surface manifestations in satellite imagery. Since tides are negligible in these seas, they can be considered non-tidal. Consequently, the main generation mechanism of IWs in the ocean-interaction of barotropic tides with bathymetry-is irrelevant. A statistically significant survey of IW occurrences in various regions of the two seas is presented. Detailed maps of spatial distribution of surface manifestations of internal waves (SMIWs) are compiled. Factors facilitating generation of IWs are determined, and a comprehensive discussion of IW generation mechanisms is presented. In the eastern and western coastal zones of the Black Sea, where large rivers disembogue, intrusions of fresh water create hydrological fronts that are able to generate IWs. At the continental shelf edge, on the west and northwest of the Black Sea and near the Crimean Peninsula, IWs are generated primarily due to relaxation of coastal upwelling and inertial oscillations associated with hydrological fronts. In addition, IWs can be formed at sea fronts associated with the passage of cold eddies. In the Caspian Sea, seiches are the main source of the observed IWs.

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