Garden asparagus, Asparagus officinalis L. (off), is dioecious and reproductively isolated from a related ornamental, monoecious Asparagus densiflorus (Kunth) Jessop cv. Sprengeri (spr). Since the latter is a potentially valuable source of germplasm, a study was initiated to identify hybridization barriers. Intra-and inter-specific crosses were made using 32 plants of two commercial cultivars of off and 17 plants of three introductions of spr. Part of the pollinated pistils were fixed and examined via fluorescence microscopy. In some combinations of genotypes, incompatibility reactions were detected: (i) off x off: in the stigmatic tissue, (ii) spr x spr: on the stigmatic surface and in the style, and (iii) spr x off: on the stigmatic surface, in the stigmatic tissue, and in the style. Although pollen tubes reached the ovules in most combinations of genotypes, seeds were only produced in intraspecific crosses. It is concluded that two types of internal barriers are acting: cross-incompatibility at the pollen-stigma and pollen-style levels, and stronger post-stylar barriers that had not been determined yet.
RCsumC: L'asperge de jardin, Asparagus ofSicinalis L. (off), est dioi'que et reproductivernent isolee d'une espkce ornementale voisine, 1'Asparagus densiflorus (Kunth) Jessop cv. Sprengeri (spr), une esptce monoi'que. Comrne cette dernitre pourait constituer une source interessante de gerrnplasme, les auteurs ont amor@ une etude pour identifier les barrikres i l'hybridation. 11s ont effectuC des croisements intra-et inter-specifiques en utilisant 32 plantes appartenant i deux cultivars commerciaux de off et 17 plants de trois introductions de spr. Une partie des pistils pollinises ont Ctt fixes et examines par rnicroscopie en fluorescence. Dans certaines combinaisons de gtnotypes, ils ont observe des reactions d'incornpatibilitC : (i) off x off: dans les tissus des stigmates, (ii) spr x spr : sur la surface des stigmates et dans le style, et (iii) spr x off: sur la surface des stigmates, dans les tissus des stigmates, et dans le style.Bien que des tubes polliniques atteignent les ovules dans la plupart des combinaisons de genotypes, les graines ne sont produites que dans les croisements intra-sptcifiques. Les auteurs concluent que les deux types de barrikres internes agissent : incornpatibilitt de croisement au niveaux pollen-stigmate et pollen-style, et qu'il y a des barritres post-stylaires plus fortes que ce qui avait t t C determine jusqu'ici.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.