We present a genome assembly from an individual male Bombus hypnorum (the Tree Bumblebee; Arthropoda; Insecta; Hymenoptera; Apidae). The genome sequence is 297 megabases in span. Most of the assembly is scaffolded into 12 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 15.6 kilobases in length. Ensembl rapid annotation of this assembly identified 13,622 protein coding genes.

We present a genome assembly from an individual female Bombus terrestris (the Buff-tailed Bumblebee; Arthropoda; Insecta; Hymenoptera; Apidae). The genome sequence is 393.0 megabases in span. Most of the assembly is scaffolded into 18 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 24.7 kilobases in length. Gene annotation of this assembly on Ensembl identified 14,435 protein coding genes.

We present a genome assembly from an individual female Eristalinus sepulchralis (the small spotty-eyed dronefly; Arthropoda; Insecta; Diptera; Syrphidae). The genome sequence is 376.8 megabases in span. Most of the assembly is scaffolded into 6 chromosomal pseudomolecules, including the X sex chromosome. The mitochondrial genome has also been assembled and is 15.96 kilobases in length. Gene annotation of this assembly on Ensembl identified 21,752 protein coding genes.

The Darwin Tree of Life (DToL) project aims to sequence and assemble high-quality genomes from all eukaryote species in Britain and Ireland, with the first phase of the project concentrating on family-level coverage plus species of particular ecological, biomedical or evolutionary interest. We summarise the processes involved in (1) assessing the UK arthropod fauna and the status of individual species on UK lists; (2) prioritising and collecting species for initial genome sequencing; (3) handling methods to ensure that high-quality genomic DNA is preserved; and (4) compiling standard operating procedures for processing specimens for genome sequencing, identification verification and voucher specimen curation. We briefly explore some lessons learned from the pilot phase of DToL and the impact of the Covid-19 pandemic.

We present a genome assembly from an individual female Bombus pascuorum (the Common Carder Bee; Arthropoda; Insecta; Hymenoptera; Apidae). The genome sequence is 307.5 megabases in span. Most of the assembly is scaffolded into 17 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 21.9 kilobases in length. Gene annotation of this assembly on Ensembl identified 12,999 protein coding genes.