Аннотация. Представлены результаты кариологического изучения двух сибирских видов Nitraria (N. sibirica и N. schoberi) из разных популяций Южной Сибири. Кариотипы этих видов характеризуются миксо-плоидией и вариабельностью хромосомных чисел при основном числе х=12. У N. sibirica чаще встречается диплоидное число хромосом 2n=24, у N. schoberi преобладает тетраплоидное число 2n=48.Ключевые слова: селитрянка сибирская, селитрянка Шобера, число хромосом, кариотип. Summary. Results of karyological study of two Nitraria species (N. sibirica and N. schoberi) from different populations of South Siberia are presented. Karyotypes of these species are characterized by mixoploidy and chromosome numbers variability on the base x=12. In N. sibirica diploid chromosome number 2n=24 dominates, in N. schoberi tetraploid chromosome number 2n=48 prevails.
We present the results of cytogenetic analysis of seven young and long-term cultured embryogenic cell lines (CLs) of Larix sibirica obtained by somatic embryogenesis. All CLs were found to be mixoploid. Three CLs (1, 2 and 6 years of cultivation) were the most stable cytogenetically with 91-96% cells having diploid chromosome number 2n=24, typical for the species. These CLs can be successfully used for the development of planting material and in clonal plantation forestry. At the same time, some embryogenic CLs were shown to possess various chromosome and genome mutations. We found two aneuploidy proliferating CLs (2n=25 and 28) capable of somatic embryo formation. One of the CLs analyzed in a current study was found to be cytogenetically unstable with chromosome numbers ranging from 24 to 35 and containing one-two micronuclei in the interphase cells. Fluorescent in situ hybridization (FISH) and morphometric analyses of karyotypes of these embryogenic CLs allow an assumption that the trisomy for one or several chromosomes is the most widespread type of genomic disturbances in embryogenic CLs of L. sibirica.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.