Background: It is estimated that the burden of care for stroke patients' care providers is increasing in the world due to the lack of support from the organizations and associations. This study aimed to evaluate the effect of health education about care of stroke patients on the burden of care providers and their quality of life. Setting: Neurology Department for nursing staff and stroke out patients' clinic for family care providers at Tanta University Hospitals. Design: A quasi-experimental. Subject: All nurses 50 from Neurology Department and convenience sample 70 of family care providers. Tools: 6 tools were used: Tool (I) Nursing Interview Questionnaire. Tool (II) Expanded Nursing Stress Scale. Tool (III) Family Care providers Interview. Tool (IV) Zarit Burden Questionnaire. Tool (V) Ways of Coping Questionnaire. Tool (VI) Quality of Life (SF-36) Questionnaire. Results: Immediately after implementation of the educational program; (62.0%) of the studied nurses had occasionally stress factors, and (72.0%) of them had increase in quality of life. As well as (67.1%) of the family care providers had mild to moderate burden and (54.3%) of them had increase in quality of life. Conclusion: Immediately after implementation of the educational program there was an enhancement in the studied nurses and family care providers’ knowledge, using of coping ways, and quality of life and reduction in the care of burden. Recommendation: Further study is recommended on large sample and in different hospitals settings in order to generalize the result.