IN THE PRESENT study, we first aimed to select some potential anti-cancer probiotics in vitro. Using the inulin fermentation product by the selected five probiotic bacteria (Lactobacillus paraplantarum strain S143, L. pentosus strain P2-3, L. plantarum strain J12, L.gasseri strain F. and L.reuteri strain IRT), Caco-2 cells were exposed to 50% filtrate supernatant of each probiotic bacteria after 24 h of incubation. Results showed that inulin fermentation product of the selected probiotics cause apoptosis in the Caco-2 cell line. Also, the viability of the freezedried powders of the five probiotics using skimmed milk as cryoprotectant was followed up for 12 weeks of storage at refrigerated conditions and also at room temperature. Results proved that lyophilized powders showed slight decrease in their viable count during storage both at room and refrigerated temperatures. This would ensure a good cell concentration for at least 3 months to be administered as a probiotic and would guarantee the long-term delivery of sufficient active, viable, and functional cultures.