Non-small cell lung cancer has been shown to be resistant to many forms of chemotherapy and is amongst the deadliest cancers worldwide. The antiproliferative effects of halogenated boroxine -K 2 (B 3 O 3 F 4 OH), have been confirmed in multiple cancer cell lines including melanoma and breast cancer. The potential for this chemical treatment on non-small cell lung cancer cells was studied and the lower threshold concentration with the clear biological effect of halogenated boroxine, was determined. Appling MTT assays and the relative gene expression analysis of two genes of interest, RRBP1 and PER1, novel knowledge on the biological potential of halogenated boroxine (HB) was gained, but did not lead to biological explanations of the mechanisms of halogenated boroxine activity. The results of MTT assay showed a significant HB effect on non-small cell lung cancer in concentration of 0.5 mg/mL, while relative expression levels of RRBP1 and PER1 did not significantly differ regardless the concentration applied.