The plant secondary metabolites (PSMs) are novel sources for the development of pharmaceuticals, food additives, and other industrial products. Plants are a ubiquitous source of potential novel medicinal compounds. In recent years, the commercial value of these secondary metabolites has sparked a lot of interest in their production and finding ways to improve it using tissue culture technologies. Secondary metabolites of the plant are currently the focus of lot of research, but extracting them for phytochemical or biological studies poses unique obstacles that must be handled throughout the extraction process. A thorough evaluation of the applicable literature for which procedures are suited for a given class of chemicals or plant species, is essential for successful extraction. Secondary metabolites are bioactive compounds recognized for healing a variety of ailments and possessing antioxidant properties, which leads to the isolation of new and unique compounds. This review gives an in-detail overview of extraction, screening, and analysis of the plant secondary metabolites concerning their bioactivity.