Introduction: Medical waste is generated by healthcare facilities such as hospitals, clinics, blood banks, and laboratories, which may cause infection to any person coming into contact with it. therefore, medical waste can be considered as being of the greatest environmental concern since it can harbor potentially harmful microorganisms and carries the risk of transmission of infections from healthcare facilities to healthcare workers, patients, and general public. Method: This cross sectional descriptive study was carried out in Almak Nimer University Hospitals - River Nile state -Sudan during the year 2021 with the aim to assess knowledge, attitudes and practices among the health care personnel towards medical waste management. Total 125 respondents were participated in the study; the study uses questionnaires, interviews and observations as tools for data collection. The collected data were analyzed by Software Package for Social Sciences (SPSS) and the result was presented in figures and tables. Result: A total of 125 subjects participated in this study which included ( 34 doctors, 56 nurse, 18 lab technician, 17 class IV employees). The study revealed that (96.8%) of the respondents have good knowledge about hazardous waste. (94.4 % )of the respondents have good knowledge concerning waste transmitting diseases. ( 84.08%) have good attitudes towards BMW ,the study most of participant want to upgrade knowledge of BMW management. about practices (77.6%) wearing personal protective measure while handling BMW during the work .( 96%) wash their hands after the work, but only (61%) use drugs for cleaning the hand if injured. Conclusion: The study revealed that level of knowledge over all (73.5%), there is good knowledge among health worker except class IV which is found to be reasonable. There are reasonable level of knowledge by class IV regarding define color-coding system. The attitude support BMW management is a team work which is found to be good as well as attitude of upgrading knowledge is found to be good. Some of participant had not received vaccination , there is good practice toward handling of BMW as well as have good practice about using special container for disposal.