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This study examined the effects of cryopreservation on DNA integrity of spermatozoa from 34 fertile subjects and 166 infertile subjects comprised of 80 teratospermic, 32 normospermic, 30 astheno-teratospermic, and 24 oligo-astheno-teratospermic individuals. Semen samples were prepared by swim-up and the Percoll density gradient centrifugation method (Pdgc) prior to freezing in liquid nitrogen. Neat and prepared samples were supplemented with cryoprotectant (SpermFreez) in cryoampoules and were frozen using the static phase vapor cooling procedure. Sperm DNA integrity of all thawed samples was determined using the alkaline comet assay. It was noticed that the sperm DNA integrity of frozen samples of fertile subjects was considerably higher than that of infertile subjects with greater catch-up integrity similar to the fresh samples. Freezing caused less chromatin damage to sperm of Pdgc samples from both fertile and infertile subjects as was compared to the neat and swim-up samples. It is concluded that the increase in comet frequency of frozen-thawed samples from infertile subjects was more prominent (8.25-22.78%; P50.01) than in the fresh samples. Frozen-thawed samples from Ts (Teratospermic individuals) and ATs (Astheno-teratozoosspermic) showed higher level of OTM (Olive tail moment) indicating a higher level of chromatin fragmentation than fertile, Ns (Normospermics), and OATs (Oligo-astheno-teratozoospermics).

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Objective: Objective of this study was to determine internet addiction and its impact on mental health and academic performance in medical students. Methodology: One hundred medical students (male: 50, female: 50) aged 18–21 years were selected by convenience sampling in this cross-sectional study in Mahi-ud-din Islamic Medical college Mirpur AJK. A questionnaire of Young’s internet addiction test was used to identify the prevalence of Internet addiction, the purposes of Internet use, and their priority levels. Frequencies and level of mental health was measured using Mental health Battery by Singh and Gupta. Chi square test was applied and p value <0.05 was considered significant. Results: Out of 100 students, 46 (male: 28, female: 18) were found to be slightly addicted, 53 students were average online users and only 01 male was severely addicted. There was no significant difference between male and female students in addiction level. However, males were more addicted than females. The major use of Internet was to communicate with friends and family and to watch songs and movies. 51 students used the Internet to assess information for their educational and learning activities. Some students with overuse of the Internet lead to insufficient sleep and affected their concentration levels in the class. Conclusion: Medical students experiencing problems due to Internet overuse and experiencing poor academic progress and lack of concentration while studying. The main use of the Internet was to communicate with friends and family and for entertainment.

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