This study was conducted to determine the antibacterial effect of crude and fractionated extracts of leaves of Vernonia amygdalina against multiple antibiotic resistant bacteria isolate from wounds. The bacteria isolates obtained are: Staphylococcus aureus, Escherichia coli, Pseudomonas aeuroginosa, Klebsiella pneumoniae, Proteus mirabilis and Streptococcus pyogene; they were confirmed standard microbiological techniques. Extracts were prepared from the leaf of the plant using ethanol, chloroform, cold water and hot water. The antibacterial activities of the crude extracts were assayed using the agar well diffusion methods. Multiple antibiotic resistant of the isolates was confirmed in the six (6) isolates. The phytochemical screening revealed the presence of alkaloids, phenols and saponins. All the extract showed varying degrees of activities depending on the extract concentration and the extract solvent. Generally, ethanol rated best as the extraction solvent which was able to inhibit all isolates except K. pneumoniae and P. mirabilis, and inhibits S. aureus at 50 mg/ml with zones 6.997±0.003. The antibacterial efficacy of the extract increases with a corresponding increase in the extract concentration. The MBC values ranged from 50 - 400 mg/mL for all the extraction solvent, and this is very important for people who depend on the plant for their health care needs. Fra 1, 6, and 10 of the column fractions were able to inhibit with 8.997 ± 0.008 mm, 8.993 ± 0.012 mm and 9.007 ± 0.000 mm respectively. The functional groups are Alkene, alkane group, alkyl halides, N-H bend of amides and Alcohol. The leaf of Vernonia amgydalina can be used as a raw material for the production of new antibiotic to control multiple resistant bacteria.
This study investigates the antifungal and antibacterial activities of extracts of Trema orientalis linn. Blume. The selected isolates used in the study includes Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella typhi, Streptococcus faecalis, Shigella dysenteriae, Proteus mirabilis, Haemolytic Streptococcus viridian, Aspergillus niger, Candida albicans ,and Aspergillus flavus. The antibiogram profile of the organism was obtained using the disc diffusion method. Tannin, flavonoid, Terpenoid, Saponin, and cardiac glycosides were found present in T. orientalis. The antibiotic sensitivity test reveals the presence of multiple antibiotic resistant bacteria. The agar well diffusion method was used to assay the antibiotic activities of the extract. The extracts were prepared from T. orientalis using Methanol and petroleum ether as extraction solvents. The antibacterial assay shows the efficacy of the methanol and petroleum ether extracts except on S. aureus ATCC 43300 and Shigella dysenteriae. The highest zones of inhibition by methanol and petroleum ether extracts were recorded on Salmonella typhi of 22.003 ± 0.003 mm and 32.002 ± 0.002 mm, respectively. The fungal isolates were susceptible to the extracts with the zones 11.67±0.33 mm and 13.67±0.33 mm. The results of the sensitivity test compared well with that of the antimicrobial test. The Minimum Inhibitory Concentration ranged between 3.125- 25.00 mg/mL. T. Orientalis contains bioactive compounds that has antimicrobial effects. The leaf of Trema orientalis has broad - spectrum activity, hence T. Orientalis methanol and Petroleum ether extracts is recommended for exploration as source of antimicrobial agents.
This study investigated the extent of urban prosperity (Infrastructural Development) using residents' perceptions in selected settlements (Ota and Agbado) of Ogun State, Nigeria. The study adopted the survey research design (majorly questionnaire). The sample frame consisted of 4,375 households in Ota (Otun) and 3,312 households in Oke-Aro (Agbado Core) which translates to 7,687 households. The sample size was 722 households' heads. The method of data analysis included frequency tables and likert scale outputs through Statistical Package for Social Science (SPSS). Based on the findings, core residential area has majority (73%) of dwellers as indigenes while 54% were holders of secondary certificates. The study found that 49.3% were self-employed as the major (56%) housing type design was bungalow. From the Likert scale outputs, the study revealed that the extent of urban prosperity in terms of infrastructure (public Service) were health infrastructure with 3.36 Residents' Perception Index (RPI), public school standard with 3.25 RPI and road transport with 3.06 RPI, Also, the extent of urban prosperity in terms of infrastructures (Housing) with built as planned has 3.95 RPI and housing affordability with 3.62 RPI respectively. It was concluded that, urban settlements (Otun in Ota and Oke-Aro in Agbado) have tendency of being urban prosperous centers considering the RPI for policy formulation.
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