4-Methylimidazole (4-MEI) is a color widely found in cola drinks, roasted foods, grilled meats, coffee and other foods. This study was aimed to investigate the 4-MEI effects on the cell proliferation, purifi ed circular DNA and DNA from cells of rats treated with the 4-MEI. In this study, mouse 3T3-L1 cell line was treated with 4-MEI at concentrations of 300, 450, 600 and 750 μg/mL for 24 hours and 48 hours periods, after that cytotoxic effect of the 4-MEI was studied by MTT test. Also, the effect of 4-MEI on purifi ed circular DNA (pET22b) was investigated by treating of the DNA with 4-MEI concentrations of 300, 450, 600 and 750 μg/ml. DNA was extracted from liver cells of rats that have been treated with 4-MEI doses of 25 and 50 mg/kg for 10 week and it was subjected to agarose gel electrophoreses analyses.4-MEI signifi cantly inhibited cell proliferation of 3T3-L1 cell line at highest concentration for 24 h and at all concentration for 48 h treatment time. DNA fragmentation assay showed that 4-MEI at 50 mg/kg concentration clearly produced characteristic DNA smear and no DNA laddering (200bp) was observed when mouse was exposed to 4-MEI. The results obtained from plasmid DNA damaging assay showed that 4-MEI has noeffect on the DNA, because the electrophoretic pattern of DNA treated with 4-MEI showed three bands on agarose gel electrophoresis as it was for untreated control. 4-MEI showed cytotoxic effect on 3T3-L1 cells but no effect on plasmid DNA breaking. According to DNA fragmentation assay 4-MEI has necrosis effects on mouse liver cells (Tab. 1, Fig. 4, Ref. 27). Text in PDF www.elis.sk.

4-methylimidazole is widely used in pharmaceuticals, photographic and agricultural chemicals. The substance is extensively found in many human and animals foods. In this research, anticancer effect of the 4-MEI was studded using MTT test using MCF-7 cell line. Effect of the 4-MEI on apoptosis or necrosis was analyzed by DNA fragmentation assay using Swiss Albino rats as a model organism. Antioxidant effect of the substance was investigated by assaying protective effect of the substance on circular plasmid DNA against H 2 O 2 as an oxidative agent. 4-MEI showed inhibitory effect on proliferation of MCF-7 cell line by all concentrations and the decrement was signifi cant and concentration dependent. Result of DNA fragmentation assay showed 4-MEI concentrations dependent of smear formation showing necrotic effects of the 4-MEI on mouse cells. Also, the 4-MEI showed a good antioxidant activity and protective effect against H 2 O 2. CONCLUSION: The result of this study showed that 4MEI has signifi cant antioxidant and anti-cancer effect. Also, according to the result, 4-MEI has necrotic effects on mouse cells (Fig.

4-Methylimidazole (4-MEI) is mostly used in beverages and coloring food, dark beers and common brands of cola drinks, which may contain more than 100 μg of this compound per 12-ounce serving. This study was aimed to investigate the antigenotoxic and anticytotoxic effects of 4-MEI (100, 130 and 160 mg/kg) against ethyl methanesulfonate (240 mg/kg) using chromosome aberrations (CAs) and Mitotic index (MI) tests in bone marrow cells of Swiss Albino Mice at 12 h and 24 h treatment periods. So, the t-test was used for the statistical analysis. In this research, 4-MEI at all concentrations for 12 h treatment period reduced chromosomal aberrations and at 130 and 160 mg/kg concentrations for 24 h treatment period increased chromosomal aberrations induced by EMS (240 mg/kg), but th ese reductions and increases were not signifi cant. Also, intraperitoneal injection of 4-MEI at doses of 100, 130 and 160 mg/kg combined with EMS (240 mg/kg) showed that the mitotic index was decreased at 100 and 130 mg/kg for 12h and 130 mg/kg for 24 h treatment periods, when compared to positive sample (EMS), but did not show any statistically difference from the EMS treated group. It can be concluded that 4-MEI might not be antigenotoxic and protective effects in bone marrow cells of Swiss Albino Mice, because 4-MEI could not reduce the chromosomal aberrations induced by EMS (Tab. 2, Fig. 2, Ref. 36). Text in PDF www.elis.sk.

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Coronavirus (Wuhan Coronavirus) is a type of virus which has RNA and determined by club like spikes of its surface. The virus is a pathogen virus and it can be infected humans and other animals. This study was aimed to design and analysis of multi epitope vaccine against Wuhan Coronavirus by different bioinformatics analysis and software's.The vaxiJen score of these sequences were 0.4052, 0.4201, and 0.4371. The result of Allg Pred and Toxinpred showed that the vaccine does not have allergenic or toxic effect for human cells. The result obtained from Protparam showed that the molecular weight of vaccine is 48.68 kDa. The estimated half-life of multi epitope based vaccine was found to be greater than 20 hours in mammalian cells, greater than 30 hours in yeast cells, and greater than 10 hours in E.coli and the candidate vaccine is stable and soluble in water.The membrane helices value of vaccine was 17.73%. Ramachandran plot analysis supported the high quality structure of the refined model. The result of protein protein docking showed the maximum affinity of candidate vaccine to HLA-A0201, HLA -B2705, HLA-B5704, and HLA -B57037 with the score of -686.54,-709.05, -633.53, and 642.61 respectively. The result of this research showed that our designed vaccine maybe stimulate B-Cells and different types of T-cells. However, the vaccine could be produced (in lab scale) and tested in laboratory animals.