In order to determine the fungi that association with ocular infections of bovine in Diyala/ Iraq. Eighty samples were collected from bovine which shows clinical signs of inflammation of the eye in the calves and cows are profuse Altadma, watery, serous and purulent thick secretions of the eye, swelling eyelids, adhesion eyelashes, keratitis with Keratohelcosis, congivetitis keratitis, emaciation and weakness. These samples were collected during four months, which began in January 2018 till April, from several areas of Baqubah city these samples were collected by sterile cotton swabs from the eyes and conjunctiva sac. The results showed that (45%) bovine samples were positive fungal isolation. The result showed higher percentage of fungal isolated in February(30%) followed by January(35%), March(25%) and April showed lower percentages of fungal isolates(10%).The current results explained that most of eye infections were unilateral (80%) while the bilateral were (20%). In conclusions, the fungus is one of the most important reasons that lead to eye infection and inflammation. The main fungal species that isolated were Aspergillus spp.(50%), followed by Mucorales also isolated (22.3%) then Candida spp. (16.6%), as well as the lower percentage of Alterneria spp. (11.1%).