Background The aim of this work is to present the results of a comparative study between the ALT assay on Integra 400 plus Roche Diagnostic versus Architect ci8200 of Abbott Diagnostic. Methods A total of 200 patients hospitalized in the various departments of the university hospital Mohammed VI of Oujda were prospectively tested on two systems: Abbott Architect ci8200 and Roche Integra 400 plus. Both analyzers use the spectrophotometric technique by coupling the transamination reaction to the oxidation-reduction reaction at NAD. The agreement of the results between the different techniques was evaluated using the Bland-Altman difference diagram and the Passing-Bablok and Deming regression line. Results There was a high concordance between the two assays: the equation of the Passing-Bablok line is YArchitect = −0,5625 + 0,9917 XIntegra with a correlation coefficient r 2 = 0.999. The Bland-Altman diagram shows that the mean bias between the two methods is of the order of 1.1 IU/L and the difference between the ALT measurements by Architect and Integra is in the range of −1.4 to 3.6. Conclusion Our study shows a high correlation of the ALT assay results between the architect ci8200 and Integra 400 plus.
L'électrophorèse des protéines sériques (EPS) est une analyse de routine dans la pratique quotidienne d'un laboratoire de biologie médicale. Le but de notre étude est d’analyser les différents profils électrophorétiques rencontrés dans notre pratique courante. Nous avons mené une étude transversale portant sur 410 échantillons sériques qui ont été collectés dans le cadre de l’analyse de routine au sein du laboratoire de biochimie du Centre Hospitalier Universitaire (CHU) Mohammed VI d’Oujda. L’électrophorèse des protéines sériques a été réalisée sur automate Capilarys2 Flex piercing de Sebia. 241 sérums de femmes et 169 sérums d’hommes ont été collectés. Âgés de 1 à 91 ans avec une moyenne d’âge de 49 ans. 19,5% des EPS étaient normales, l’hypo-albuminémie a été noté chez 34% des cas, un syndrome inflammatoire chronique dans 19,5% des cas, un syndrome néphrotique chez 2% des cas, 5,8% de nos patients avaient un bloc béta-gamma, une hypogammaglobulinémie a été observée dans 8,5% des cas, et 29 pics monoclonaux ont été notés, une bis-albuminémie a été objectivée chez 2 patients. Sur les 410 prélèvements: 92 immunofixations ont été réalisées dont 23 étaient positives (objectivant une gammapathie monoclonale). Cette étude met en évidence la variabilité des motifs de prescription d’une électrophorèse des protéines sériques, ainsi que l’importance des données cliniques pour une interprétation meilleure.
Pasteurella multocida can cause serious infections after dog or cat bite. We report here a rare case of hand infection caused by P. multocida consecutive to an injury by a thorn of the prickly pear. It caused an amputation of the distal phalanx of the thumb in a trisomic patient.It is about a 27-year-old man who was admitted to the hospital with swelling and intense pain of the left hand. He reported a sting by a thorn of prickly pear 15 days before. The patient was admitted to proceed with operative irrigation and debridement. The pus was collected for microbiological examination. Microscopic examination after Gram staining revealed small Gram-negative coccobacilli, associated to polymorphonuclear reaction. Culture have objectivated Pasteurella multocida. The isolated strain was susceptible to betalactamins. Patient was treated with ampicillin. Well-conducted antibiotics and repetitive local cares have not prevented local lesions from progressing to necrosis of the soft parts of the thumb and osteitis of the distal phalanx of the thumb. The patient underwent a necrosectomy and an amputation of the distal phalanx. Ampicillin was replaced by amoxicillin/clavulanic acid and after 15 days, progression was clinically and microbiologically favorable.In the case we report, since the patient does not report any exposure or contact with animals, the thorn prick is the source of infection. It was contaminated from the animal reservoir. Taking into account the monomicrobism of the infection, treatment with aminopenicillins was sufficient. Our propositus came to the hospital 15 days after the inoculation of the bacterium. This duration appears to be very late in relation to the acute character of pasteurellosis. This was probably the main reason why the local infection evolved towards osteoarticular complications. That's why, we should consider Pasteurella multocida in case of infection by inoculation, even in the absence of contact with the animals.
Data regarding the quantification of CD34+ hematopoietic stem cell HSC in dual-platform (DP) approach using a hematological analyzer featuring dedicated nRBC channel are limited if not inexistent. The objective of this study is to compare the performance of the DP approach on quantification of CD34+ HSC using a new generation of hematological analyzer featuring dedicated nRBC channel, Sysmex XN-1000, with the single-plate (SP) form approach using Beckman Coulter's Navios flow cytometer. Using the International Society for Hematotherapy and Transplantation protocol, a total of 86 samples were analyzed in multiple myeloma patients planned for autologous HSC transplantation. The Beckman Coulter's Stem-Kit™ reagents were used to perform both approaches DP and SP. The count of CD34+ cells of the SP deduced directly from the software that manage the Navios flow cytometry was compared to CD34+ cell count obtained by DP. The DP approach was made by combining flow cytometry analysis and the Sysmex XN-1000 hematological cell analyzer data. The analysis of the agreement of the results between these two approaches using the Bland-Altman diagram gave a bias of one cell/μl. As for the equation of the right of Passing-Bablok, it is Y (SP) = 0.166667 + 0.969697 X (DP). The slight differences in CD34+ stem cell counting between these two approaches did not achieve statistical significance. Both SP and DP approaches were effective and yield similar results. DP seems to be a simple and affordable approach that is well suited to the needs of laboratories where this type of analyzer is available.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2025 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.