In clinical doses, propofol strongly attenuates lipid peroxidation during CABG surgery.

In this study, we report data on the possible genotoxic effect of inhalation anesthetic sevoflurane (SVF) by comparing two techniques, comet and alkaline halo assay, in peripheral blood lymphocytes (PBL) of patients before, during, and after anesthesia and in controls. DNA single strand breaks were detected in PBL of malignant breast cancer diagnosed patients (stage II-III), who were undergoing mastectomy. Blood samples were taken before the induction of anesthesia, at 120 min of SVF anesthesia, and on the postoperative fifth day. The nuclear spreading factor (NSF) for each cell was assessed by alkaline halo assay, and the total comet score (TCS) was evaluated by comet assay. A statistically significant increase (P < 0.0001) was observed in the mean NSF at 120 min of anesthesia (38.24 +/- 14.14) as compared with samples before anesthesia (12.33 +/- 6.14), and the mean NSF was significantly decreased after the postoperative fifth day (17.89 +/- 9.44). Similar results were obtained by the comet assay with significant increase (P < 0.0001) in DNA damage at 120 min of anesthesia (79.66 +/- 15.28) as compared with samples before anesthesia (36.30 +/- 11.39). The DNA damage was almost with the preoperative damage rates after the fifth day of anesthesia (43.40 +/- 12.19). In conclusion, the study points out a reversible genotoxic effect of SVF and the similar DNA damage levels obtained by comet and alkaline halo assay indicate that although halo assay has a completely different principle, it can conveniently be utilized for the assessment of DNA single strand breakage in individual mammalian cells with its experimental advantages.

Malignant hyperthermia is a hypermetabolic response to inhalation agents (such as halothane, sevoflurane, and desflurane), succinylcholine, vigorous exercise, and heat. Reactions develop more frequently in males than females (2 : 1). The classical signs of malignant hyperthermia are hyperthermia, tachycardia, tachypnea, increased carbon dioxide production, increased oxygen consumption, acidosis, muscle rigidity and rhabdomyolysis. In this case report, we present a case of delayed onset malignant hyperthermia-like reaction after the second exposure to sevoflurane.

In the present state of knowledge, anaesthesia and surgery depress immune system by direct effects of anesthetic agents and indirect interaction of hormonal and metabolic changes caused by surgical stress. Every component of immunologic profile can be altered during anaesthesia and sur- - Hematological and most of the immunological measurements were in normal ranges throughout the study and no statistically significant difference was observed between sevoflurane and isoflurane groups. Time related differences observed in each group consisting increases in IgG, B and T lymphocytes and T-helper and decrease in T-suppressor levels with protection of complement levels in isoflurane group. Although the laboratory data showed better immune response for isoflurane, statistical analyses did not support these findings. Even though different aspects of immune system changes have been in the advantage of isoflurane regarding laboratory data, statistical methods did not revealed a meaningful difference in each comparison.K Ke ey y W Wo or rd ds s: Immune Response, Isoflurane, Minor Surgery, Sevoflurane, Ö ÖZ ZE ET T M Mi in nö ör r C Ce er rr ra ah hi i G Gi ir ri iş şi im ml le er rd de e İ İs so of fl lo or ra an n v ve e S Se ev vo of fl lo or ra an nı ın n İ İm mm mü ün n S Si is st te em me e E Et tk ki il le er ri i Anestezi ve cerrahinin immün sisteme olan etkileri uzun süredir bilinmektedir ve immün etkileşim girişimlerin morbiditesini arttırabilmektedir. Prospektif çalışmamızda sık kullanılan inhalasyon anestetikleri sevofloran ve izofloranın minör cerrahi uygulanan hastalarda immunolojik etkilerini karşılaştırmayı planladık. İmmünolojik yetmezliği olmayan ve minör cerrahi girişim uygulanan 44 hasta çalışmaya dahil edildi.Aynı indüksiyon tekniği uygulandıktan sonra 22 hastaya izofloran ve 22 hastaya sevofloran uygulandı.Kan örnekleri preoperatif ve postoperatif 1 ve 3. Günlerde olmak üzere 3 kez alındı. Laboratuvar incelemelerde lökosit sayımı, serum immunglobulinler, kompleman, lenfositler, T-lenfosit ve alt grupları bakıldı. İzofloran ve sevofloran grupları arasında hematolojik ve çoğu immünolojik ölçümler normal sınırlar içindeydi ve istatistiksel fark yoktu.A An na ah ht ta ar r K Ke el li im me el le er r: : İmmün Yanıt, İzofloran, Minor Cerrahi, Sevofloran

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